Product Overview
dATP (100mM) is a colorless liquid solution of deoxyadenosine triphosphate, one of the four deoxynucleotide building blocks that DNA polymerases incorporate during DNA synthesis. The quality of the nucleotide matters in every enzymatic reaction that uses it: impurities can reduce polymerase efficiency, and contaminating enzymes can degrade the template or the product. This product has a purity of ≥99% by HPLC, and the solution is adjusted to pH 7.0 with high-purity NaOH solution, prepared in ultra-pure water.
Quality testing confirms that it contains no detectable DNase, RNase, or phosphatase, so it can be added directly to sensitive enzymatic reactions without additional cleanup. dATP (100mM) is suitable for a wide range of conventional molecular biology applications, including PCR amplification, real-time PCR, cDNA and general DNA synthesis, DNA sequencing, and DNA labeling.
Specifications
| Product name | Size-1 | Size-2 | Size-3 |
| dATP (100 mM) | 1 mL | 5 × 1 mL | 100 mL |
Product Features
- High purity of ≥99% (HPLC): The high-purity nucleotide supports efficient and consistent incorporation by DNA polymerases across PCR, sequencing, and DNA synthesis reactions.
- No detectable DNase, RNase, or phosphatase: Testing confirms the absence of these contaminating enzymes, protecting templates, primers, and products from degradation and the nucleotide itself from dephosphorylation.
- pH 7.0 adjusted and ready for routine reactions: The solution is pH-adjusted with high-purity NaOH, so it can be used directly in PCR and other conventional molecular biology reactions, and diluted with ultra-pure water to the needed working concentration.
FAQ
Q1: What applications is dATP (100mM) suitable for?
It is suitable for conventional molecular biology applications, including PCR amplification, real-time PCR, cDNA and general DNA synthesis, DNA sequencing, and DNA labeling.
Q2: How is the quality of this dATP verified?
The purity is ≥99% by HPLC, and the product has been tested to confirm that it contains no DNase, RNase, or phosphatase. This helps protect nucleic acid templates and products in sensitive enzymatic reactions.
Q3: Can I dilute this 100 mM stock, and does the pH need adjusting before use?
The 100 mM stock can be diluted with ultra-pure water to the working concentration required by your protocol. The solution is already adjusted to pH 7.0 with high-purity NaOH, and it can be used directly in conventional molecular biology reactions such as PCR.
