Product Overview
dNTPs are among the most basic raw materials in molecular biology, and their quality affects every enzymatic reaction that uses them. Beyond high purity, low contamination and good performance in downstream applications are what separate a dependable nucleotide mix from a merely adequate one. dNTP mix (10 mM each) mNGS is developed with this in mind, and every batch is produced according to GMP guidelines. The established QC method meets the requirements of the United States, European, and Chinese Pharmacopoeias and other regulations. The product combines dATP, dCTP, dGTP, and dTTP (trisodium salts) at 10 mM each in one colorless liquid solution, adjusted to pH 7.0 with high-purity NaOH solution.
Each component has an HPLC purity of ≥99%, and the mix is free of DNase, RNase, and phosphatase, and of contamination by qPCR, PCR, and reverse transcription inhibitors. All components have undergone strict functional quality control and meet strict requirements for DNA contamination, which is the parameter that matters most for sensitive, low-background workflows such as metagenomic sequencing. Supplying the four nucleotides pre-mixed saves pipetting steps and removes one source of variability when preparing reactions. As a general guideline, a common working concentration for PCR. The mix can also be diluted with ultra-pure water to the concentration a protocol requires. After extensive verification and validation, the product is suitable for PCR, qPCR, high-fidelity PCR, long-chain PCR, cDNA synthesis, RT-PCR, RCA, MDA, DNA markers, and DNA sequencing.
Specifications
| Product name | Size-1 | Size-2 | Size-3 |
| dNTP mix (10 mM each) mNGS | 1 mL | 5 × 1 mL | 100 mL |
Product Features
- Ready-to-use mix with ultra-high purity: dATP, dCTP, dGTP, and dTTP are pre-mixed at 10 mM each, and every component has an HPLC purity of ≥99%, which saves preparation steps and supports consistent incorporation by DNA polymerases and reverse transcriptases.
- Free of nucleases, phosphatase, and reaction inhibitors, with strict DNA contamination control: The mix is free of DNase, RNase, phosphatase, and qPCR, PCR, and RT inhibitors, and all components meet strict requirements for DNA contamination, protecting sensitive, low-background assays.
- GMP-guideline production with pharmacopoeia-level QC: Each batch is produced according to GMP guidelines, and the QC method meets the requirements of the US, European, and Chinese Pharmacopoeias, which supports the documentation needs of diagnostic and kit manufacturers.
FAQ
Q1: What does this mix contain, and what working concentration does it give?
The mix contains dATP, dCTP, dGTP, and dTTP as trisodium salts, each at 10 mM. As a general guideline, adding 1 μL to a 50 μL reaction gives 200 μM of each dNTP, a common working concentration for PCR. It can be diluted with ultra-pure water to the concentration your protocol requires. Please contact us if you need other concentrations.
Q2: What does the “mNGS” designation mean for this dNTP mix?
The mNGS version is intended for sequencing and detection workflows where background contamination matters most. It is produced under GMP guidelines, free of DNase, RNase, phosphatase, and qPCR, PCR, and RT inhibitors, with all components under strict functional quality control and meeting strict requirements for DNA contamination. Please contact us for details of the DNA contamination specification and testing method for your application.
Q3: Which applications is the mix validated for, and what quality standards does it follow?
Following verification and validation, the mix is suitable for PCR, qPCR, high-fidelity PCR, long-chain PCR, cDNA synthesis, RT-PCR, RCA, MDA, DNA markers, and DNA sequencing. Each batch is produced according to GMP guidelines, and the established QC method meets the requirements of the United States, European, and Chinese Pharmacopoeias and other regulations.
