Product Overview
HYF269 MluI Restriction Enzyme is designed for fast and efficient DNA digestion in molecular biology workflows. Its high activity in Universal Buffer and Color Buffer enables DNA digestion within 5–15 minutes under recommended reaction conditions, helping reduce processing time and simplify restriction enzyme workflows.
The enzyme is suitable for digesting plasmid DNA, genomic DNA, viral DNA, and PCR products. Its buffer compatibility also facilitates double or multiple restriction digestions in a single reaction, reducing the need for sequential digestion steps when the selected enzymes are compatible with the reaction conditions.
When used with Color Buffer, the reaction mixture can be loaded directly onto an agarose gel after digestion, eliminating the need for a separate loading dye addition step. This feature supports a more convenient workflow for routine DNA analysis and restriction digestion applications.
Components
| Components | 100 rxns |
| Velocut MluI | 100 μL |
| 10× Buffer | 1 mL |
| 10× Color Buffer | 1 mL |
Product Features
- Rapid DNA Digestion: Enables DNA digestion in 5–15 minutes under recommended reaction conditions, helping improve workflow efficiency.
- Flexible Buffer Compatibility: Performs in Universal Buffer and Color Buffer, supporting convenient restriction digestion workflows and compatible multi-enzyme reactions.
- Broad DNA Substrate Compatibility: Suitable for digestion of plasmid DNA, genomic DNA, viral DNA, and PCR products.
FAQ
Q1: How long does HYF269 MluI Restriction Enzyme take to digest DNA?
HYF269 MluI Restriction Enzyme supports DNA digestion in 5–15 minutes under recommended reaction conditions. Actual digestion time may vary depending on DNA input, reaction setup, and enzyme activity requirements.
Q2: Which types of DNA can be digested with HYF269 MluI Restriction Enzyme?
HYF269 MluI Restriction Enzyme can be used to digest plasmid DNA, genomic DNA, viral DNA, and PCR products, provided the DNA contains the appropriate recognition site.
Q3: Can HYF269 MluI Restriction Enzyme be used for double or multiple restriction digestion?
Yes. HYF269 MluI Restriction Enzyme is compatible with Universal Buffer and Color Buffer, which support convenient double or multiple restriction digestions when the selected enzymes share compatible reaction conditions. Please consult the relevant enzyme specifications and recommended reaction conditions before setting up a combined digestion.
