Product Overview
HYF277 NotI Restriction Enzyme is designed for rapid and efficient DNA digestion in molecular biology workflows. With high activity in Universal Buffer and Color Buffer, it enables DNA digestion in 5–15 minutes under recommended reaction conditions, helping streamline routine restriction digestion procedures.
The enzyme is suitable for digesting plasmid DNA, genomic DNA, viral DNA, and PCR products containing the appropriate NotI recognition site. Its buffer compatibility facilitates double or multiple restriction digestion in a single reaction tube when the selected enzymes have compatible reaction requirements, reducing the need for sequential digestion steps.
When used with Color Buffer, the digestion reaction can be loaded directly onto an agarose gel without adding a separate loading dye. The buffer contains a density reagent and red and yellow tracking dyes to facilitate sample loading and electrophoresis monitoring.
Components
| Components | 50 rxns |
| Velocut NotI | 50 μL |
| 10× Buffer | 1 mL |
| 10× Color Buffer | 1 mL |
Product Features
- Rapid DNA Digestion: Enables DNA digestion in 5–15 minutes under recommended reaction conditions, helping reduce processing time.
- Flexible Buffer Compatibility: Compatible with Universal Buffer and Color Buffer, supporting convenient single-enzyme and compatible multi-enzyme digestion workflows.
- Convenient Gel Loading: Color Buffer contains a density reagent and two tracking dyes, allowing direct loading of digestion reactions onto agarose gels without adding a separate loading dye.
FAQ
Q1: How long does HYF277 NotI Restriction Enzyme take to digest DNA?
HYF277 NotI Restriction Enzyme supports DNA digestion in 5–15 minutes under recommended reaction conditions. The actual digestion time may vary depending on the DNA substrate, DNA input, and reaction setup.
Q2: Which DNA substrates are suitable for HYF277 NotI Restriction Enzyme?
HYF277 NotI Restriction Enzyme can be used to digest plasmid DNA, genomic DNA, viral DNA, and PCR products containing the appropriate NotI recognition site.
Q3: Can HYF277 NotI Restriction Enzyme be used for double or multiple restriction digestion?
Yes. HYF277 NotI Restriction Enzyme is compatible with Universal Buffer and Color Buffer, facilitating double or multiple restriction digestion in a single reaction when the selected enzymes have compatible buffer and reaction requirements. Check the relevant enzyme specifications before combining enzymes in one reaction.
