Product Overview
HYB314 LAMP Fluorescent Master Mix is a liquid reagent system for DNA detection by loop-mediated isothermal amplification (LAMP). The system includes a Bst DNA polymerase enzyme mix, amplification buffer containing magnesium ions and dNTPs, fluorescent dye, and a lyophilization protectant.
The supplied components are combined with assay-specific primers and DNA template to establish an isothermal amplification reaction. Fluorescence measurement enables real-time monitoring of amplification on compatible instruments.
The liquid format provides flexibility for reaction setup and assay optimization. The included lyophilization protectant also supports development of freeze-dried reagent formats. HYB314 provides a platform for fluorescent DNA assay development, instrument-based molecular testing, and POCT reagent development.
Components
| Components | 96 T | 960 T | 9600 T |
| Loop-mediated Amplification Buffer | 1.2 mL | 4 mL×3 | 12 mL×10 |
| Enzymes Mix | 192 μL | 1.92 mL | 1.92 mL×10 |
| Lyophilized protectant | 0.96 mL×2 | 9.6 mL | 9.6 mL×10 |
| 25× Dye | 192 μL | 0.96 mL×2 | 0.96 mL×20 |
Product Features
- Real-Time Fluorescence Monitoring: Supports fluorescence acquisition during LAMP amplification on compatible instruments.
- Bst-Based DNA Amplification: Combines Bst DNA polymerase with amplification buffer containing magnesium ions and dNTPs for isothermal DNA amplification.
- Liquid Format with Lyophilization Development Support: Supplied as liquid components for assay setup and optimization, with a lyophilization protectant for freeze-dried formulation development.
FAQ
Q1: What is the main difference between HYB314 and HYB312?
The main difference is the detection method.
HYB312: pH-based colorimetric LAMP with visual red-to-yellow interpretation
HYB314: fluorescent LAMP with instrument-based real-time signal monitoring
HYB314 is therefore better suited for applications where objective fluorescence detection, amplification-curve monitoring, or integration with an optical POCT instrument is required.
Q2: Does HYB314 require a real-time PCR instrument?
HYB314 requires a device capable of maintaining the LAMP reaction temperature and detecting the relevant fluorescence signal.
Q3: Can HYB314 be used to develop lyophilized LAMP reagents?
Yes. HYB314 includes a lyophilization protectant that can be incorporated into the formulation when developing freeze-dried fluorescent LAMP reagents.
