Product Overview
BspQI Restriction Endonuclease recognizes the DNA sequence 5′-GCTCTTC-3′ and cleaves outside its recognition site to generate a 3-base 5′ overhang. For mRNA in vitro transcription (IVT), BspQI can be used to linearize suitably designed plasmids downstream of the poly(A) tail, helping prepare DNA templates without unwanted downstream sequences. Hyasen BspQI provides efficient DNA digestion at 50°C, with verified low nonspecific nuclease activity and ≥95% protein purity. It is suitable for plasmid linearization and DNA template preparation in mRNA research workflows.
Components
| Components | 0.5KU | 2.5KU | 10KU | 100KU | 1000KU |
| BspQI (10 U/μL) | 50 uL | 250 uL | 1 mL | 10 mL | 100 mL |
| 10×BspQI Buffer | 1.25 mL | 1.25 mL | 4 × 1.25 mL | 5 × 10 mL | 5 × 100 mL |
Product Features
- Precise Plasmid Linearization: Type IIS cleavage supports DNA template preparation with defined ends for IVT.
- Efficient DNA Digestion: Recommended reaction temperature of 50°C.
- Low Nonspecific Nuclease Activity: Quality-controlled for RNase and nonspecific DNase activity.
- High Protein Purity: ≥95% purity verified by SDS-PAGE.
FAQ
Q1: What is BspQI used for in mRNA synthesis?
BspQI is used to linearize plasmid DNA before in vitro transcription (IVT). It helps prepare linear DNA templates for downstream mRNA synthesis.
Q2: Why is BspQI used for IVT plasmid linearization?
BspQI cuts outside its recognition sequence, allowing appropriately designed plasmids to be linearized downstream of the poly(A) tail without introducing unwanted downstream sequences into the intended transcript.
Q3: How should BspQI be stored?
Store at −25°C to −15°C. The stated shelf life is 24 months under the recommended storage conditions.
