Product Overview
dNTP Mix (25 mM each) is a high-concentration, ready-to-use nucleotide mixture containing four essential deoxynucleoside triphosphates: dATP, dCTP, dGTP, and dTTP. Each nucleotide component is supplied at 25 mM, providing a convenient and flexible solution for DNA amplification and synthesis workflows that require higher concentration dNTP inputs. The balanced formulation eliminates the need for individual nucleotide preparation and mixing, helping simplify reaction setup while maintaining consistent dNTP ratios. dNTP Mix (25 mM each) can be directly incorporated into a variety of molecular biology reactions, including PCR amplification, real-time PCR, cDNA synthesis, DNA sequencing, and DNA labeling.
Each nucleotide component is manufactured with HPLC purity ≥99%, ensuring high-quality substrates for enzymatic DNA synthesis. The product is tested to be free of DNase, RNase, and phosphatase contamination, supporting reliable performance in sensitive molecular applications. Supplied as a colorless liquid solution, dNTP Mix (25 mM each) offers convenient handling and can be diluted according to specific experimental requirements. The standardized high-concentration formulation makes it suitable for routine molecular biology research, assay development, and nucleic acid amplification workflows.
Specifications
| Product name | Size-1 | Size-2 | Size-3 |
| dNTP mix (25 mM each) | 1 mL | 5 × 1 mL | 100 mL |
Product Features
- High-Concentration Four-Nucleotide Mixture for Flexible Use: dNTP Mix (25 mM each) contains dATP, dCTP, dGTP, and dTTP at equal concentrations of 25 mM, providing a convenient and flexible nucleotide source for various DNA synthesis applications.
- High Purity for Reliable Molecular Biology Applications: Each nucleotide component has an HPLC purity of ≥99%, supporting consistent performance in PCR, qPCR, sequencing, and other enzymatic DNA synthesis workflows.
- Ready-to-Use Formulation with Reduced Preparation Steps: The premixed formulation eliminates individual nucleotide preparation and mixing, helping simplify reaction setup and improve workflow consistency.
FAQ
Q1: What is included in dNTP Mix (25 mM each)?
dNTP Mix (25 mM each) contains four deoxynucleoside triphosphates: dATP, dCTP, dGTP, and dTTP. Each nucleotide is provided at a concentration of 25 mM.
Q2: What is the difference between dNTP Mix (25 mM each) and dNTP Mix (10 mM each)?
The main difference is the concentration of each nucleotide component. dNTP Mix (25 mM each) provides a higher concentration formulation, offering greater flexibility for applications requiring customized dNTP input levels or reaction optimization.
Q3: Which applications are compatible with dNTP Mix (25 mM each)?
The product is suitable for a wide range of molecular biology applications, including PCR amplification, real-time PCR, DNA synthesis, cDNA synthesis, DNA sequencing, and DNA labeling.
