Product Overview
dTTP (100 mM) is a colorless liquid solution of 2′-deoxythymidine-5′-triphosphate (trisodium salt), the thymine-containing building block that DNA polymerases incorporate opposite adenine in the template strand. Thymine is specific to DNA, which makes dTTP an essential component of any reaction that synthesizes DNA. The quality of the nucleotide matters in every enzymatic reaction that uses it: impurities can reduce polymerase efficiency, and contaminating enzymes can degrade the template or the product. This product has a purity of ≥99% by HPLC and is adjusted to pH 7.0 with high-purity NaOH solution, prepared in ultra-pure water.
Quality testing confirms that it contains no detectable DNase, RNase, or phosphatase, so it can be added directly to sensitive enzymatic reactions without additional cleanup. dTTP (100 mM) is suitable for a wide range of conventional molecular biology applications, including PCR amplification, real-time PCR, cDNA and general DNA synthesis, DNA sequencing, and DNA labeling. As a 100 mM stock, it can be diluted with ultra-pure water to the working concentration required by each protocol, and combined with the other deoxynucleotides to prepare nucleotide mixes.
Specifications
| Product name | Size-1 | Size-2 | Size-3 |
| dTTP (100 mM) | 1 mL | 5 × 1 mL | 100 mL |
Product Features
- High purity of ≥99% (HPLC): The high-purity nucleotide supports efficient and consistent incorporation by DNA polymerases across PCR, sequencing, and DNA synthesis reactions.
- No detectable DNase, RNase, or phosphatase: Testing confirms the absence of these contaminating enzymes, protecting templates, primers, and products from degradation and the nucleotide itself from dephosphorylation.
- Flexible pack sizes with a 2-year shelf life: Available in 1 mL, 5 × 1 mL, and 100 mL sizes, with a 2-year validity period when stored at -20°C or below, suitable for both routine bench use and larger-volume nucleotide mix preparation.
FAQ
Q1: How should dTTP (100 mM) be stored and handled?
Store at -20°C or below, and avoid repeated freezing and thawing. The product is shipped with ice bags and is valid for 2 years. When you need to use it, thaw it at room temperature and then keep it in an ice box or ice bath during the experiment. Return it to -20°C or below immediately after use.
Q2: What salt form is this dTTP, and how is the quality verified?
The product is the trisodium salt of 2′-deoxythymidine-5′-triphosphate (dTTP 3Na), with a molecular weight of 482.17 for the free acid. Purity is ≥99% by HPLC, and testing confirms that it contains no DNase, RNase, or phosphatase.
Q3: Can I dilute this 100 mM stock or combine it with other dNTPs to make a mix?
Yes. The 100 mM stock can be diluted with ultra-pure water to the working concentration your protocol requires, and it can be combined with the other deoxynucleotides to prepare a dNTP mix. The solution is already adjusted to pH 7.0 with high-purity NaOH, and it can be used directly in conventional molecular biology reactions such as PCR.
