Product Overview
Velocut DpnI is an engineered restriction endonuclease designed to support rapid and efficient DNA digestion in molecular biology applications. Its fast digestion performance helps reduce reaction time and simplify restriction enzyme workflows, making it suitable for routine DNA analysis and sample preparation. The enzyme is compatible with universal Buffer and Color Buffer, providing flexibility for different experimental workflows. Under suitable reaction conditions, DNA digestion can be completed within 5–15 minutes, reducing the need for extended incubation and helping improve workflow efficiency.
Velocut DpnI can be used with universal Buffer and Color Buffer. The Color Buffer contains a density reagent and red and yellow tracking dyes, allowing reaction mixtures to be loaded directly onto an agarose gel after digestion without requiring a separate loading dye. In a 1% agarose gel, the red tracking dye migrates similarly to approximately 2.5 kb double-stranded DNA fragments, while the yellow dye migrates similarly to approximately 10 bp double-stranded DNA fragments. These visual markers help users monitor electrophoresis progress during DNA fragment analysis.
Velocut DpnI is intended for DNA digestion workflows involving plasmid DNA, genomic DNA, viral DNA, and PCR products. Its rapid digestion characteristics and buffer compatibility make it useful for restriction analysis, DNA sample preparation, and other molecular biology procedures requiring enzymatic DNA cleavage. As with other restriction endonucleases, digestion performance depends on the DNA substrate, reaction conditions, and relevant methylation status. Users should select the appropriate experimental conditions based on their downstream application.
Components
| Components | 50 rxns |
| Velocut DpnI | 50 μL |
| 10× Buffer | 1 mL |
| 10× Color Buffer | 1 mL |
Product Features
- Rapid DNA Digestion: Designed to support DNA digestion within 5–15 minutes under suitable reaction conditions. The shorter incubation time can help streamline restriction digestion workflows and reduce overall experimental turnaround time.
- Compatible with Universal Buffer and Color Buffer: Works with universal Buffer and Color Buffer, offering flexibility in reaction setup. The Color Buffer contains a density reagent and two tracking dyes, allowing digested samples to be loaded directly onto an agarose gel without adding a separate loading dye.
- Broad DNA Substrate Applications: Suitable for digestion workflows involving plasmid DNA, genomic DNA, viral DNA, and PCR products. Velocut DpnI can be incorporated into molecular biology workflows requiring restriction enzyme activity, subject to substrate compatibility and appropriate reaction conditions.
FAQ
Q1: What are the main applications of Velocut DpnI?
Velocut DpnI is used for restriction digestion in molecular biology workflows involving plasmid DNA, genomic DNA, viral DNA, and PCR products. It can support DNA sample preparation and restriction analysis where the enzyme’s recognition specificity and substrate requirements are appropriate.
Q2: How long does Velocut DpnI take to digest DNA?
Velocut restriction enzymes are designed to digest DNA within approximately 5–15 minutes under suitable reaction conditions. The actual digestion time may vary depending on the DNA substrate, enzyme amount, DNA input, and reaction setup. For reliable results, follow the product-specific protocol and confirm that the selected conditions are appropriate for the target DNA.
Q3: What is the difference between universal Buffer and Color Buffer?
Both buffers are designed to support restriction digestion. Color Buffer additionally contains a density reagent and red and yellow tracking dyes, allowing the digestion reaction to be loaded directly onto an agarose gel without adding a separate loading dye. In a 1% agarose gel, the red dye migrates similarly to approximately 2.5 kb double-stranded DNA fragments, while the yellow dye migrates similarly to approximately 10 bp double-stranded DNA fragments.
