Product Overview
Velocut HpaII (HYF265) is designed to support efficient DNA digestion in molecular biology applications. As part of the Velocut restriction enzyme series, it offers rapid digestion performance, with DNA digestion achievable in 5–15 minutes under suitable reaction conditions. This shorter incubation time can help improve workflow efficiency in routine DNA analysis and molecular cloning procedures.
Velocut HpaII is compatible with both universal Buffer and Color Buffer, providing flexibility for restriction digestion workflows. The Color Buffer contains a density reagent and red and yellow tracking dyes, allowing reaction mixtures to be loaded directly onto an agarose gel without adding a separate loading dye. In a 1% agarose gel, the red tracking dye migrates similarly to approximately 2.5 kb double-stranded DNA fragments, while the yellow dye migrates similarly to approximately 10 bp double-stranded DNA fragments. These visual markers help monitor electrophoresis progress.
Velocut HpaII can be used to digest plasmid DNA, genomic DNA, viral DNA, and PCR products. It is suitable for DNA digestion workflows supporting molecular cloning, restriction fragment analysis, and other molecular biology procedures that require restriction enzyme treatment. The Velocut enzyme series is designed to enable rapid digestion and, where enzyme compatibility and reaction conditions permit, simultaneous digestion with multiple restriction enzymes in a single reaction. For HpaII-specific recognition, methylation sensitivity, and reaction conditions, consult the product datasheet before setting up experiments.anning a digestion workflow.
Components
| Components | 200 rxns |
| Velocut HpaII | 200 μL |
| 10× Buffer | 2×1 mL |
| 10× Color Buffer | 2×1 mL |
Product Features
- Rapid DNA Digestion: Enables DNA digestion in 5–15 minutes under suitable reaction conditions, helping reduce incubation time and improve workflow efficiency.
- Compatible with Universal Buffer and Color Buffer: Supports flexible reaction setup, while Color Buffer enables direct loading of reaction mixtures onto agarose gels without adding a separate loading dye.
- Suitable for Multiple DNA Substrates: Supports digestion of plasmid DNA, genomic DNA, viral DNA, and PCR products for applications such as molecular cloning and restriction analysis.
FAQ
Q1: What is Velocut HpaII used for?
Velocut HpaII is a restriction enzyme used for DNA digestion in molecular biology workflows. It can be used with plasmid DNA, genomic DNA, viral DNA, and PCR products. For HpaII-specific applications, recognition-site details, and methylation-related considerations, refer to the product datasheet.
Q2: How long does Velocut HpaII take to digest DNA?
Velocut enzymes are designed to digest DNA in approximately 5–15 minutes under suitable reaction conditions. The actual incubation time depends on the DNA substrate, enzyme amount, and recommended reaction setup. Follow the product datasheet for the appropriate conditions for Velocut HpaII.
Q3: Can Velocut HpaII be used with Color Buffer?
Yes. Velocut HpaII is part of the Velocut enzyme series, which is designed for activity in both universal Buffer and Color Buffer. Color Buffer contains a density reagent and tracking dyes, allowing the reaction mixture to be loaded directly onto an agarose gel. Confirm the recommended buffer and reaction conditions in the product datasheet.
