Product Overview
Velocut KpnI (HYF267) is designed to support efficient DNA digestion in molecular biology workflows. As part of the Velocut restriction enzyme series, it enables DNA digestion in 5–15 minutes under suitable reaction conditions, helping reduce incubation time and streamline routine laboratory procedures.
Velocut KpnI is compatible with both universal Buffer and Color Buffer, offering flexibility in restriction digestion workflows. Color Buffer contains a density reagent and red and yellow tracking dyes, allowing reaction mixtures to be loaded directly onto an agarose gel without adding a separate loading dye. In a 1% agarose gel, the red tracking dye migrates similarly to approximately 2.5 kb double-stranded DNA fragments, while the yellow tracking dye migrates similarly to approximately 10 bp double-stranded DNA fragments. These tracking dyes help monitor electrophoresis progress during DNA analysis.
Velocut KpnI can be used to digest plasmid DNA, genomic DNA, viral DNA, and PCR products. It supports restriction digestion workflows for molecular cloning, DNA fragment analysis, and other molecular biology applications. For workflows involving multiple restriction enzymes, simultaneous digestion may be possible when the enzymes have compatible reaction requirements. Refer to the product datasheet for KpnI-specific information, including its recognition sequence, recommended reaction conditions, and methylation sensitivity.
Components
| Components | 200 rxns |
| Velocut KpnI | 200 μL |
| 10× Buffer | 2× 1 mL |
| 10× Color Buffer | 2× 1 mL |
Product Features
- Rapid DNA Digestion: Enables DNA digestion in 5–15 minutes under suitable reaction conditions, helping reduce incubation time and improve laboratory workflow efficiency.
- Compatible with Universal Buffer and Color Buffer: Supports flexible reaction setup, while Color Buffer allows direct loading of reaction mixtures onto agarose gels without adding a separate loading dye.
- Suitable for Multiple DNA Substrates: Supports digestion of plasmid DNA, genomic DNA, viral DNA, and PCR products for molecular cloning, DNA analysis, and related research applications.
FAQ
Q1: What is Velocut KpnI (HYF267) used for?
Velocut KpnI (HYF267) is a restriction enzyme used to digest DNA for molecular biology applications, including molecular cloning and DNA fragment analysis. It is suitable for workflows involving plasmid DNA, genomic DNA, viral DNA, and PCR products.
Q2: How long does Velocut KpnI (HYF267) take to digest DNA?
Velocut KpnI (HYF267) supports rapid DNA digestion in approximately 5–15 minutes under suitable reaction conditions. Actual digestion time depends on the DNA substrate, enzyme amount, and reaction setup. Follow the product datasheet for recommended conditions.
Q3: Is Velocut KpnI (HYF267) compatible with Color Buffer?
Yes. Velocut KpnI (HYF267) is compatible with universal Buffer and Color Buffer. Color Buffer contains a density reagent and tracking dyes, allowing the reaction mixture to be loaded directly onto an agarose gel without adding a separate loading dye.
