Product Overview
Velocut XcmI (HYF412) is a recombinant restriction endonuclease designed for rapid DNA digestion in molecular biology workflows. It is suitable for plasmid DNA, PCR products, and genomic DNA containing the specific recognition site. Under the recommended conditions, digestion can be completed in 5–15 minutes at 37°C using a 1× reaction buffer.
The supplied color reaction buffer contains red and yellow tracking dyes, allowing digestion products to be loaded directly onto an agarose gel without adding a separate loading dye. In a 1% agarose gel, the red dye migrates similarly to a 2,500 bp double-stranded DNA fragment, while the yellow dye migrates similarly to a 10 bp fragment.
Velocut XcmI can be heat-inactivated at 80°C for 20 minutes, providing a convenient option for workflows that require enzyme inactivation before subsequent processing.
Product Features
- Rapid DNA Digestion: Completes digestion in 5–15 minutes at 37°C under the recommended reaction conditions.
- Convenient Direct Gel Loading: The supplied color reaction buffer contains red and yellow tracking dyes for direct loading of digestion products onto agarose gels.
- Reliable Heat Inactivation: Can be heat-inactivated at 80°C for 20 minutes to support downstream molecular biology workflows.
FAQ
Q1: How long does Velocut XcmI take to digest DNA?
Velocut XcmI typically completes digestion in 5–15 minutes at 37°C using the recommended 1× reaction buffer. Actual performance may depend on the DNA substrate and reaction setup.
Q2: Can Velocut XcmI be heat-inactivated after digestion?
Yes. Velocut XcmI can be heat-inactivated by incubating the reaction at 80°C for 20 minutes.
Q3: Can Velocut XcmI digestion products be loaded directly onto an agarose gel?
Yes. The supplied color reaction buffer contains red and yellow tracking dyes, allowing digestion products to be loaded directly onto an agarose gel without adding a separate loading dye.
