RT-LAMP Colormetric Master Mix（Lyophilized beads） HYB412
This product contains reaction buffer, RT- Enzymes Mix (Bst DNA polymerase and heatresistant reverse transcriptase), lyophilized protectants and chromogenic dye components. The product is freeze-dried ball type, using only with primers and templatesThis kit provides a fast, clear visual detection of amplification, which negative reaction is indicated in red and positive reaction is indicated by a change to yellow.
Components
|
Components |
96 T |
960 T |
9600 T |
|
RT-LAMP Colormetric Master Mix （Lyophilized beads） |
8 T/strip×12 |
8 T/strip×120 |
8 T/strip×1200 |
Storage
Store at 2-8℃, valid for 12 months.
Instructions
1. Take out the corresponding number Lyophilized beads powder according to the number of tests.
2. Prepare reaction mix
|
Components |
Volume |
|
RT-LAMP Colormetric Master Mix （Lyophilized beads） |
1 piece（2 beads） |
|
10×Primer Mix* |
5 μ L |
|
Template DNA/RNA** |
45 μ L |
*10×Primer Mix Concentration: 16 μM FIP/BIP, 2 μM F3/B3,4 μM Loop F/B;
**Nucleic acid templates are recommended to be dissolved using DEPC water.
3. Incubate at 65°C for 30-45min, which can beextended appropriately according to color change Reaction time.
4. According to the naked eye, yellow was positiveand red was negative.
Notes
1. The reaction temperature can be optimizedbetween 62 °C and 68 °C according to the primer conditions.
2. The packaged reagents should not be exposedto air for a long time.
3. The red and yellow discoloration reactiondepend on the pH change of the reaction system, please do not use the containing Tris nucleic acid storage solution,recommended to use ddH2O stored nucleic acid;
4. The experiment shall be conducted in astandardized manner, including the preparation of reaction system, sample treatment and sample
5. It is suggested to prepare reaction system inthe ultra-clean table and add templates in the fume hood of other rooms to avoid false.