Product Overview

HYB412 RT-LAMP Colorimetric Master Mix (Lyophilized Beads) is a freeze-dried reagent system for isothermal amplification of RNA and DNA targets with visual endpoint detection. Each bead combines reaction buffer, Bst DNA polymerase, a heat-resistant reverse transcriptase, colorimetric detection components, and lyophilization protectants.

For RNA targets, reverse transcription and LAMP amplification take place in a single reaction. DNA templates can be amplified directly by Bst DNA polymerase. Following incubation under the specified assay conditions, positive reactions change from red to yellow, while negative reactions remain red. Results are interpreted visually at the defined endpoint alongside appropriate positive and negative controls, without a fluorescence reader.

The beads are reconstituted and combined with assay-specific primers and nucleic acid template before use. This format reduces liquid reagent preparation and supports the development of visual molecular assays, POCT workflows, and freeze-dried assay kits.

Components

Product Features

• Ready-to-Use Lyophilized Beads for Simplified Assay Setup: HYB412 packages the core RT-LAMP reaction chemistry into a standardized freeze-dried bead format.
• One-Step RT-LAMP for RNA and DNA Detection: HYB412 contains both Bst DNA polymerase and a heat-resistant reverse transcriptase, supporting one-step isothermal amplification from RNA templates as well as direct DNA amplification.
• Clear Red-to-Yellow Visual Readout: HYB412 incorporates chromogenic components for simple endpoint interpretation.

FAQ