Product Overview
HYB414 RT-LAMP Fluorescent Master Mix (Lyophilized Beads) is a freeze-dried reagent system designed for RNA detection by reverse transcription loop-mediated isothermal amplification (RT-LAMP). Each bead combines reaction buffer, Bst DNA polymerase, a heat-resistant reverse transcriptase, fluorescent dye, and lyophilization protectants, reducing liquid reagent preparation and simplifying reaction setup.
The formulation enables reverse transcription and strand-displacement amplification in a single reaction under isothermal conditions. Amplification can be monitored in real time using a compatible fluorescence detection instrument.
After reconstitution and addition of assay-specific primers and RNA template, the beads provide a convenient format for establishing fluorescent RT-LAMP assays. The preformed beads also facilitate reagent handling and integration into assay consumables.
Combining one-step RNA amplification with real-time fluorescence monitoring, HYB414 supports molecular assay development and reagent integration for point-of-care testing (POCT) platforms.
Components
| Components | 96 T | 960 T | 9600 T |
| RT-LAMP Fluorescent Master Mix(Lyophilized beads) | 8 T/strip×12 | 8 T/strip×120 | 8 T/strip×1200 |
Product Features
- Ready-to-Use Lyophilized Beads for Flexible Assay Integration: HYB414 packages the core RT-LAMP reaction chemistry into standardized freeze-dried beads.
- One-Step RT-LAMP with Real-Time Fluorescent Detection: HYB414 combines reverse transcription and Bst-mediated isothermal amplification in a single reaction system.
- Freeze-Dried Format for POCT and Commercial Diagnostic Development: HYB414 is supplied in a stabilized lyophilized bead format, making it particularly suitable for ready-to-use molecular diagnostic products.
FAQ
Q1: What is the main difference between HYB414 and HYB413 RT-LAMP Fluorescent Master Mix?
Both products are designed for fluorescent RT-LAMP RNA detection, but their reagent formats are different.
HYB413: liquid / lyophilization-ready RT-LAMP fluorescent master mix
HYB414: pre-manufactured lyophilized bead format
Q2: What needs to be added before using HYB414?
HYB414 already contains the core RT-LAMP amplification and fluorescence detection chemistry in a freeze-dried bead.
Q3: Why choose lyophilized beads instead of in-situ lyophilization?
The main advantage is packaging flexibility.
