RT-LAMP Fluorescent Master Mix (Lyophilized beads） HYB414
This product contains reaction buffer, RT-Enzymes Mix for Bst DNA polymerase and heat-resistant reverse transcriptase, lyophilization protector and fluorescent dye components. The reaction buffer contains Mg2+, dNTP and other components necessary for amplification. The product is lyophilized in pellet form, and only primers and templates need to be added for use.
Components
|
Components |
96 T |
960 T |
9600 T |
|
RT-LAMP Fluorenscent Master Mix（Lyophilized beads） |
8 T/strip×12 |
8 T/strip×120 |
8 T/strip×1200 |
Storage
Store at 2-8℃, valid for 12 months.
Instructions
1. Take out the corresponding number Lyophilized beads powder according to the number of tests.
2. Prepare reaction mix
|
Components |
Volume |
|
RT-LAMP Fluorenscent Master Mix （Lyophilized beads） |
1 piece（2 beads） |
|
10×Primer Mix* |
5 μ L |
|
Template DNA/RNA** |
45 μ L |
*10×Primer Mix Concentration: 16 μM FIP/BIP, 2 μM F3/B3,4 μM Loop F/B;
**Nucleic acid templates are recommended to be dissolved using DEPC water.
3. Reaction program
Set the parameters on the PCR instrument (such as ABI7500) as follows:
|
Temperature |
Time |
Cycles |
|
65℃ |
60s *Collect fluorescent sign |
30 |
Notes
1. The reaction temperature can be optimized between 62°C and 68°C according to the primer conditions.
2. The experiment shall be conducted in a standardized manner, including the preparation of reaction system, sample treatment and sample addition.
3. It is suggested to prepare reaction system in the ultra-clean table and add templates in the fume hood of other rooms to avoid false.