Product Overview
Respiratory and other clinical specimens present a particular challenge for pathogen testing. Sputum, BALF, and ascites are complex liquid matrices, and the microbial nucleic acids of interest are often present at low levels among a large background of host material, so an extraction method that purifies and enriches the target is important for reliable detection. The Magnetic Pathogen DNA/RNA Kit is designed for the purification and enrichment of pathogen DNA and RNA, including viruses, bacteria, and fungi, from biological liquid specimens such as sputum, bronchoalveolar lavage fluid (BALF), blood, and ascites. Covering several pathogen types in one kit is convenient for laboratories that run broad pathogen screening or investigate infections of unknown cause. Because the kit uses a magnetic bead format, separation is performed with a magnet instead of centrifugation or vacuum filtration, which simplifies handling and makes the workflow easier to scale to larger sample numbers. The microbial nucleic acids purified with this kit are compatible with a full range of downstream assays, including PCR, RT-qPCR, real-time fluorescent quantitative PCR, and next-generation sequencing (NGS), supporting both targeted detection and sequencing-based approaches.
Components
| Components | 96 T |
| Buffer LBS-A | 20 mL |
| Buffer ML | 25 mL |
| Buffer GW1 | 180 mL |
| Buffer GW2 | 180 mL |
| RNase-Free Water | 10 mL |
| Proteinase K | 4×1.25 mL |
| Magbeads PN | 2×1.5 mL |
| Lysis Tubes II | 96 |
Product Features
- Purification and enrichment of viral, bacterial, and fungal nucleic acids: One kit covers the DNA and RNA of viruses, bacteria, and fungi, supporting laboratories that screen for multiple pathogen types from the same specimen.
- Designed for challenging clinical liquid specimens: The kit is designed for sputum, BALF, blood, and ascites, which are complex matrices common in respiratory and infectious disease testing.
- Compatible with targeted and sequencing-based assays: Purified nucleic acids are compatible with PCR, RT-qPCR, real-time fluorescent quantitative PCR, and NGS, so one extraction can support both targeted detection and sequencing workflows.
FAQ
Q1: Which specimen types is this kit designed for?
The kit is designed for biological liquid specimens including sputum, bronchoalveolar lavage fluid (BALF), blood, and ascites.
Q2: Which types of pathogens can this kit purify?
The kit purifies and enriches pathogen DNA and RNA from viruses, bacteria, fungi, and other microbes, so it is suitable for laboratories that need to test for more than one type of pathogen from the same specimen.
Q3: What downstream applications is the purified nucleic acid suitable for?
The microbial nucleic acids are compatible with a full range of downstream assays, including PCR, RT-qPCR, real-time fluorescent quantitative PCR, and next-generation sequencing (NGS).
