Product Overview
HYF223 AccI Restriction Enzyme is designed for rapid and efficient DNA digestion in molecular biology workflows. Under recommended reaction conditions, it can complete DNA digestion in 5–15 minutes at 37°C, helping streamline routine restriction enzyme procedures.
HYF223 AccI Restriction Enzyme is suitable for digesting plasmid DNA, PCR products, and genomic DNA containing the appropriate AccI recognition site. Its compatibility with standard and color reaction buffers supports convenient restriction digestion and combined workflows when the selected enzymes and downstream reagents have compatible reaction requirements.
The color reaction buffer contains a density reagent and red and yellow tracking dyes, allowing digestion reactions to be loaded directly onto an agarose gel without adding a separate loading dye. In a 1% agarose gel, the red dye migrates similarly to approximately 2,500 bp double-stranded DNA fragments, while the yellow dye migrates similarly to approximately 10 bp double-stranded DNA fragments.
HYF223 AccI Restriction Enzyme can be heat-inactivated by incubation at 80°C for 20 minutes. Cleavage may be blocked or impaired when the substrate DNA is methylated by CpG methylation. When planning a digestion workflow, consider the methylation status of the DNA substrate and follow the recommended reaction conditions.
Product Features
- Rapid DNA Digestion: Enables DNA digestion in 5–15 minutes at 37°C under recommended reaction conditions, supporting efficient routine molecular biology workflows.
- Convenient Gel Loading: The color reaction buffer contains a density reagent and two tracking dyes, allowing digestion reactions to be loaded directly onto agarose gels without a separate loading dye.
- Defined Inactivation Conditions: Can be heat-inactivated at 80°C for 20 minutes. DNA methylation by CpG methylation may inhibit or impair cleavage, making substrate methylation status an important consideration during experimental planning.
FAQ
Q1: How long does HYF223 AccI Restriction Enzyme take to digest DNA?
Under recommended reaction conditions, DNA digestion can typically be completed in 5–15 minutes at 37°C. Follow the product protocol for the appropriate reaction setup and DNA input requirements.
Q2: Can DNA methylation affect HYF223 AccI Restriction Enzyme activity?
Yes. Cleavage may be blocked or impaired when the substrate DNA is methylated by CpG methylation. If digestion is incomplete, consider the methylation status of the DNA and verify the recommended reaction conditions.
Q3: Can the digestion reaction be loaded directly onto an agarose gel?
Yes, when the color reaction buffer is used. It contains a density reagent and red and yellow tracking dyes, allowing direct gel loading without adding a separate loading dye. The enzyme can also be heat-inactivated at 80°C for 20 minutes when required by the downstream workflow.
