Product Overview
BsiWI (HYF211) is a Type IIP restriction endonuclease designed for sequence-specific DNA digestion in molecular biology workflows. It recognizes palindromic DNA sequences and uses an optimized reaction buffer to support enzyme activity. The reaction buffer contains recombinant albumin, which helps improve enzyme stability.
For recommended use, BsiWI is incubated at 55°C in the supplied 1× reaction buffer. The enzyme can be heat-inactivated at 80°C for 5 minutes. Cleavage of CpG-methylated DNA may be inhibited, so the methylation status of the DNA substrate should be considered when planning digestion experiments.
Product Features
- Palindromic Sequence Recognition: A Type IIP restriction endonuclease for sequence-specific cleavage of palindromic DNA targets.
- Optimized Reaction Buffer: The supplied reaction buffer contains recombinant albumin to help improve enzyme stability.
- Defined Reaction and Inactivation Conditions: Operates at 55°C and can be heat-inactivated at 80°C for 5 minutes. Cleavage of CpG-methylated DNA may be inhibited.
FAQ
Q1: What type of restriction enzyme is BsiWI?
BsiWI is a Type IIP restriction endonuclease that recognizes palindromic DNA sequences.
Q2: What are the recommended reaction conditions for BsiWI?
Use the recommended 1× reaction buffer and incubate at 55°C. Prepare the reaction according to the applicable DNA digestion protocol.
Q3: Does CpG methylation affect BsiWI digestion?
Cleavage of CpG-methylated DNA may be inhibited. Consider the methylation status of the DNA substrate when planning the digestion workflow.
