Product Overview
Nt.BspQI (HYF419) is a nicking endonuclease designed for DNA manipulation workflows that require single-strand cleavage. It acts on double-stranded DNA by cutting only one strand, leaving the complementary strand intact rather than producing a double-strand break.
The recommended reaction conditions are 1× Cut Buffer C at 50°C. Nt.BspQI also exhibits 100% activity at 37°C under the specified assay conditions and can be heat-inactivated at 80°C for 5 minutes.
Cleavage of EcoBI-methylated DNA may be inhibited. The methylation status of the DNA substrate should therefore be considered when planning experiments.
Product Features
- Single-Strand DNA Nicking: Cleaves only one strand of dsDNA without cutting both strands.
- Flexible Reaction Temperature: Recommended for use at 50°C, with 100% activity at 37°C under the specified assay conditions.
- Defined Inactivation Conditions: Can be heat-inactivated at 80°C for 5 minutes. Cleavage of EcoBI-methylated DNA may be inhibited.
FAQ
Q1: What is the function of Nt.BspQI?
Nt.BspQI is a nicking endonuclease that cuts only one strand of double-stranded DNA, creating a single-strand nick without cleaving both strands.
Q2: What are the recommended reaction conditions for Nt.BspQI?
Use 1× Cut Buffer C and incubate at 50°C. The enzyme also exhibits 100% activity at 37°C under the specified assay conditions and can be heat-inactivated at 80°C for 5 minutes.
Q3: Can DNA methylation affect Nt.BspQI activity?
Yes. Cleavage of EcoBI-methylated DNA may be inhibited. Consider the methylation status of the DNA substrate when planning the reaction.
