Product Overview

HYF233 Velocut AvrII is a recombinant restriction endonuclease developed for rapid and efficient DNA digestion. Under recommended reaction conditions, it can complete digestion within 5–15 minutes at 37°C, helping streamline molecular biology workflows.

HYF233 Velocut AvrII is suitable for digesting DNA substrates containing the appropriate AvrII recognition site, including plasmid DNA, PCR products, and genomic DNA. It is compatible with standard reaction buffer and color reaction buffer. The color buffer contains red and yellow tracking dyes, allowing digestion products to be loaded directly onto an agarose gel without adding a separate loading dye. On a 1% agarose gel, the red and yellow dyes migrate similarly to approximately 2,500 bp and 10 bp double-stranded DNA fragments, respectively.

HYF233 Velocut AvrII cannot be heat-inactivated. After digestion, remove the enzyme using phenol-chloroform extraction or a suitable DNA purification column, following the applicable protocol. Store the enzyme at −20°C. The stated shelf life is two years under the recommended storage conditions.

Product Features

• Rapid DNA Digestion: Completes DNA digestion in 5–15 minutes at 37°C under recommended reaction conditions, supporting efficient restriction digestion workflows.
• Convenient Gel Loading: Compatible with color reaction buffer containing red and yellow tracking dyes, allowing digestion products to be loaded directly onto agarose gels without a separate loading dye.
• Non-Heat-Inactivatable Enzyme: Cannot be heat-inactivated. Remove the enzyme after digestion by phenol-chloroform extraction or DNA purification column cleanup, as appropriate for the downstream workflow.

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