Product Overview
HYF216 Velocut BsaI is a recombinant restriction endonuclease developed for rapid DNA digestion in molecular biology workflows. It can be used to digest DNA containing the appropriate BsaI recognition site, including plasmid DNA, PCR products, and genomic DNA.
Under the recommended reaction conditions, digestion can be completed in 5–15 minutes at 37°C. The enzyme is compatible with standard reaction buffer and color reaction buffer. The color reaction buffer contains red and yellow tracking dyes, allowing digested samples to be loaded directly onto an agarose gel without adding a separate loading dye. In a 1% agarose gel, the red dye migrates similarly to approximately 2,500 bp double-stranded DNA, while the yellow dye migrates similarly to approximately 10 bp double-stranded DNA.
Cleavage by HYF216 Velocut BsaI may be inhibited by CpG or Dcm methylation. The enzyme can be heat-inactivated at 80°C for 20 minutes and stored at −20°C for up to 2 years.
Product Features
- Rapid DNA Digestion: Enables DNA digestion in 5–15 minutes at 37°C under recommended reaction conditions.
- Convenient Gel Analysis: Color reaction buffer contains tracking dyes, allowing digested samples to be loaded directly onto agarose gels.
- Defined Reaction and Storage Conditions: Can be heat-inactivated at 80°C for 20 minutes; store at −20°C for up to 2 years. CpG or Dcm methylation may inhibit cleavage.
FAQ
Q1: How long does HYF216 Velocut BsaI take to digest DNA?
HYF216 Velocut BsaI can complete digestion in 5–15 minutes at 37°C under the recommended reaction conditions. Actual performance may depend on the DNA substrate and reaction setup.
Q2: Can DNA methylation affect HYF216 Velocut BsaI activity?
Yes. Cleavage may be inhibited by CpG or Dcm methylation. Consider the methylation status of your DNA when planning a digestion reaction.
Q3: How should HYF216 Velocut BsaI be inactivated and stored?
HYF216 Velocut BsaI can be heat-inactivated at 80°C for 20 minutes. Store the enzyme at −20°C for up to 2 years.
