Product Overview

HYF245 Velocut Cfr42I (SacII) is a recombinant restriction endonuclease designed for efficient digestion of DNA containing the appropriate recognition site. It is suitable for applications involving plasmid DNA, PCR products, and genomic DNA, with reactions typically completed in 5–15 minutes at 37°C under the recommended buffer conditions.

For added convenience, a color reaction buffer containing red and yellow tracking dyes allows digested samples to be loaded directly onto an agarose gel without adding a separate loading dye. In a 1% agarose gel, the red dye migrates similarly to approximately 2,500 bp double-stranded DNA, while the yellow dye migrates similarly to approximately 10 bp double-stranded DNA.

Cleavage of CpG-methylated DNA may be inhibited, so the methylation status of the DNA substrate should be considered when planning digestion. The enzyme can be heat-inactivated at 80°C for 20 minutes and should be stored at −20°C, with a shelf life of two years.

Product Features

• Rapid DNA Digestion: Completes restriction digestion in 5–15 minutes at 37°C under recommended reaction conditions.
• Convenient Gel Electrophoresis: A color reaction buffer with red and yellow tracking dyes enables direct loading of digestion reactions onto agarose gels.
• Defined Inactivation and Storage Conditions: Heat-inactivates at 80°C for 20 minutes and can be stored at −20°C for two years.

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