Product Overview
HYF249 Velocut DpnII is a recombinant restriction endonuclease designed for efficient digestion of DNA containing the appropriate DpnII recognition site. It is suitable for restriction digestion workflows involving plasmid DNA, PCR products, and genomic DNA. Under the recommended reaction conditions, digestion can typically be completed in 5–15 minutes at 37°C.
For convenient downstream analysis, a color reaction buffer containing red and yellow tracking dyes allows digestion reactions to be loaded directly onto an agarose gel without adding a separate loading dye. In a 1% agarose gel, the red dye migrates similarly to approximately 2,500 bp double-stranded DNA, while the yellow dye migrates similarly to approximately 10 bp double-stranded DNA.
Cleavage of Dam- or EcoBI-methylated DNA may be inhibited, so the methylation status of the DNA substrate should be considered when planning digestion. HYF249 Velocut DpnII can be heat-inactivated at 80°C for 20 minutes and should be stored at −20°C, with a shelf life of two years.
Product Features
- Rapid DNA Digestion: Completes restriction digestion in 5–15 minutes at 37°C under recommended reaction conditions.
- Convenient Gel Electrophoresis: A color reaction buffer with red and yellow tracking dyes enables direct loading of digestion reactions onto agarose gels.
- Defined Methylation Sensitivity and Inactivation: Heat-inactivates at 80°C for 20 minutes and can be stored at −20°C for two years.
FAQ
Q1: How long does HYF249 Velocut DpnII take to digest DNA?
A: Under the recommended reaction conditions, digestion is typically completed in 5–15 minutes at 37°C.
Q2: Can HYF249 Velocut DpnII digest Dam- or EcoBI-methylated DNA?
Cleavage of DNA methylated by Dam or EcoBI may be inhibited. Consider the methylation status of the DNA substrate when planning your digestion workflow.
Q3: How should HYF249 Velocut DpnII be inactivated and stored?
The enzyme can be heat-inactivated at 80°C for 20 minutes. Store at −20°C; the stated shelf life is two years.
