Product Overview
HYF252 Velocut EagI is a recombinant restriction endonuclease developed for efficient DNA digestion in molecular biology workflows. It is suitable for digesting plasmid DNA, PCR products, and genomic DNA containing the appropriate EagI recognition site. Under recommended reaction conditions, digestion can typically be completed in 5–15 minutes at 37°C.
For convenient downstream analysis, a color reaction buffer containing red and yellow tracking dyes allows digestion reactions to be loaded directly onto an agarose gel without adding a separate loading dye. In a 1% agarose gel, the red dye migrates similarly to approximately 2,500 bp double-stranded DNA, while the yellow dye migrates similarly to approximately 10 bp double-stranded DNA.
Cleavage of CpG-methylated DNA may be inhibited, so the methylation status of the DNA substrate should be considered when planning digestion. HYF252 Velocut EagI can be heat-inactivated at 80°C for 20 minutes.
Product Features
- Rapid DNA Digestion: Supports restriction digestion in 5–15 minutes at 37°C under recommended reaction conditions.
- Convenient Gel Electrophoresis: A color reaction buffer with red and yellow tracking dyes enables direct loading of digestion reactions onto agarose gels.
- Defined Heat Inactivation: Can be heat-inactivated at 80°C for 20 minutes, supporting convenient downstream workflow planning.
FAQ
Q1: How long does HYF252 Velocut EagI take to digest DNA?
Under the recommended reaction conditions, digestion is typically completed in 5–15 minutes at 37°C.
Q2: Does CpG methylation affect HYF252 Velocut EagI activity?
Cleavage of CpG-methylated DNA may be inhibited. Consider the methylation status of your DNA substrate when planning the digestion workflow.
Q3: Can HYF252 Velocut EagI be heat-inactivated after digestion?
Yes. HYF252 Velocut EagI can be heat-inactivated at 80°C for 20 minutes.
