Product Overview

HYF257 Velocut FokI is a recombinant restriction endonuclease developed for efficient DNA digestion in molecular biology workflows. It is suitable for digesting plasmid DNA, PCR products, and genomic DNA containing the appropriate FokI recognition site. Under recommended reaction conditions, digestion can typically be completed in 5–15 minutes at 37°C.

For convenient downstream analysis, a color reaction buffer containing red and yellow tracking dyes allows digestion reactions to be loaded directly onto an agarose gel without adding a separate loading dye. In a 1% agarose gel, the red dye migrates similarly to approximately 2,500 bp double-stranded DNA, while the yellow dye migrates similarly to approximately 10 bp double-stranded DNA.

Cleavage of Dcm-, CpG-, or EcoBI-methylated DNA may be inhibited, so the methylation status of the DNA substrate should be considered when planning digestion. HYF257 Velocut FokI can be heat-inactivated at 80°C for 20 minutes and should be stored at −20°C, with a shelf life of two years.

Product Features

• Rapid DNA Digestion: Supports restriction digestion in 5–15 minutes at 37°C under recommended reaction conditions.
• Convenient Gel Electrophoresis: A color reaction buffer with red and yellow tracking dyes enables direct loading of digestion reactions onto agarose gels.
• Defined Inactivation and Storage Conditions: Cleavage may be inhibited by Dcm, CpG, or EcoBI methylation. The enzyme can be heat-inactivated at 80°C for 20 minutes.

FAQ