Product Overview
HYF259 Velocut HaeIII is a recombinant restriction endonuclease developed for rapid and efficient DNA digestion. It is suitable for plasmid DNA, PCR products, and genomic DNA containing the appropriate recognition site.
Under recommended reaction conditions, Velocut HaeIII completes digestion in 5–15 minutes at 37°C. This short incubation time helps streamline routine DNA preparation workflows. The enzyme is supplied with reaction buffers, including a color buffer containing red and yellow tracking dyes for direct loading of digestion products onto agarose gels. In a 1% agarose gel, the red dye migrates similarly to approximately 2,500 bp double-stranded DNA, while the yellow dye migrates similarly to approximately 10 bp double-stranded DNA.
After digestion, the enzyme can be heat-inactivated at 80°C for 20 minutes. Velocut HaeIII is suitable for molecular biology workflows requiring rapid restriction digestion and convenient downstream gel analysis.
Product Features
- Rapid DNA Digestion: Completes restriction digestion in 5–15 minutes at 37°C under recommended reaction conditions, helping improve workflow efficiency.
- Convenient Gel Loading: The supplied color reaction buffer contains red and yellow tracking dyes, allowing digestion products to be loaded directly onto agarose gels without adding a separate loading dye.
- Reliable Heat Inactivation: Can be heat-inactivated at 80°C for 20 minutes, simplifying post-digestion handling and downstream processing.
FAQ
Q1: How long does Velocut HaeIII take to digest DNA?
Velocut HaeIII typically completes digestion in 5–15 minutes at 37°C when used with the recommended reaction buffer and conditions.
Q2: Which DNA substrates are suitable for Velocut HaeIII?
Velocut HaeIII is suitable for plasmid DNA, PCR products, and genomic DNA containing its recognition site.
Q3: Can Velocut HaeIII be heat-inactivated after digestion?
Yes. Velocut HaeIII can be heat-inactivated by incubation at 80°C for 20 minutes.
