Product Overview
HYF271 Velocut MscI is a rapid restriction enzyme developed for efficient DNA digestion in molecular biology workflows. Under the recommended reaction conditions, it can digest suitable DNA substrates in 5–15 minutes at 37°C, helping streamline DNA preparation for downstream applications.
The supplied color reaction buffer contains red and yellow tracking dyes, allowing digested samples to be loaded directly onto an agarose gel without adding a separate loading dye. In a 1% agarose gel, the red dye migrates similarly to approximately 2,500 bp double-stranded DNA, while the yellow dye migrates similarly to approximately 10 bp double-stranded DNA.
The enzyme can be heat-inactivated at 80°C for 20 minutes. Cleavage of Dcm-methylated DNA may be inhibited, so methylation status should be considered when designing digestion workflows. Store at −20°C for up to 2 years.
Product Features
- Rapid DNA Digestion: Completes restriction digestion in 5–15 minutes at 37°C under the recommended reaction conditions, supporting efficient processing of plasmid DNA, PCR products, and genomic DNA containing the recognition site.
- Convenient Gel Electrophoresis: The supplied color reaction buffer contains red and yellow tracking dyes, enabling direct gel loading and easier monitoring of electrophoresis progress.
- Defined Inactivation and Methylation Profile: Heat-inactivates at 80°C for 20 minutes. Digestion of Dcm-methylated DNA may be inhibited and should be considered during experimental planning.
FAQ
Q1: How long does Velocut MscI digestion take?
Velocut MscI typically completes digestion in 5–15 minutes at 37°C under the recommended reaction conditions. Actual digestion performance depends on the DNA substrate and reaction setup.
Q2: Can Velocut MscI digest Dcm-methylated DNA?
Cleavage of Dcm-methylated DNA may be inhibited. Consider the methylation status of the DNA substrate when planning the digestion reaction.
Q3: Can the digestion product be loaded directly onto an agarose gel?
Yes. The supplied color reaction buffer contains red and yellow tracking dyes, allowing direct gel loading. In a 1% agarose gel, the red dye migrates similarly to approximately 2,500 bp double-stranded DNA, and the yellow dye migrates similarly to approximately 10 bp double-stranded DNA.
