Product Overview
HYF278 Velocut NruI is a rapid restriction enzyme developed to support efficient DNA digestion in molecular biology workflows. Under the recommended reaction conditions, it enables digestion in 5–15 minutes at 37°C, helping streamline DNA preparation for downstream applications.
The supplied color reaction buffer contains red and yellow tracking dyes, allowing digestion products to be loaded directly onto an agarose gel without adding a separate loading dye. In a 1% agarose gel, the red dye migrates similarly to approximately 2,500 bp double-stranded DNA, while the yellow dye migrates similarly to approximately 10 bp double-stranded DNA.
Velocut NruI cannot be heat-inactivated. Phenol-chloroform extraction or column purification is recommended when enzyme removal is required after digestion. Cleavage of Dam- or CpG-methylated DNA may be inhibited, so the methylation status of the substrate should be considered during experimental planning. Store at −20°C for up to 2 years.
Product Features
- Rapid DNA Digestion: Supports DNA digestion in 5–15 minutes at 37°C under the recommended reaction conditions for suitable plasmid DNA, PCR products, and genomic DNA containing the recognition site.
- Direct Gel Loading: The supplied color reaction buffer contains red and yellow tracking dyes, allowing direct gel loading and convenient monitoring of electrophoresis progress.
- Methylation Awareness and Post-Digestion Purification: Cleavage of Dam- or CpG-methylated DNA may be inhibited. The enzyme cannot be heat-inactivated, so phenol-chloroform extraction or column purification is recommended when enzyme removal is required.
FAQ
Q1: How long does Velocut NruI digestion take?
Under the recommended reaction conditions, Velocut NruI typically completes DNA digestion in 5–15 minutes at 37°C. Actual performance depends on the DNA substrate and reaction setup.
Q2: Can Dam or CpG methylation affect Velocut NruI digestion?
Yes. Cleavage of Dam- or CpG-methylated DNA may be inhibited. Consider the methylation status of the DNA substrate when planning the digestion reaction.
Q3: Can Velocut NruI be heat-inactivated after digestion?
No. Velocut NruI cannot be heat-inactivated. Phenol-chloroform extraction or column purification is recommended when removal of the enzyme is required after digestion.
