Product Overview

Velocut PvuII is a recombinant restriction endonuclease designed for rapid DNA digestion in molecular biology workflows. It supports the digestion of plasmid DNA, PCR products, and genomic DNA containing the PvuII recognition site, with reactions typically completed in 5–15 minutes at 37°C using the recommended buffer conditions.

The supplied color reaction buffer contains red and yellow tracking dyes, allowing digested DNA samples to be loaded directly onto agarose gels without adding a separate loading dye. In a 1% agarose gel, the red dye migrates similarly to approximately 2,500 bp double-stranded DNA, while the yellow dye migrates similarly to approximately 10 bp double-stranded DNA.

Velocut PvuII cannot be heat-inactivated. When enzyme removal is required before downstream applications, phenol-chloroform extraction or column purification is recommended. Cleavage of EcoBI-methylated DNA may be inhibited.

Product Features

• Rapid DNA Digestion: Completes restriction digestion in 5–15 minutes at 37°C, supporting efficient workflows for plasmid DNA, PCR products, and genomic DNA containing the target recognition site.
• Direct Gel Loading: The color reaction buffer contains red and yellow tracking dyes, enabling direct agarose gel electrophoresis and convenient monitoring of sample migration.
• Defined Reaction Compatibility: Suitable for standard restriction digestion workflows, with EcoBI methylation sensitivity and the need for post-digestion purification considered during experimental planning.

FAQ