Product Overview

Velocut RsaI is a recombinant restriction endonuclease designed for rapid DNA digestion in molecular biology workflows. It is suitable for plasmid DNA, PCR products, and genomic DNA containing the RsaI recognition site. Under the recommended conditions with 1× reaction buffer at 37°C, digestion can typically be completed in 5–15 minutes.

The supplied color reaction buffer contains red and yellow tracking dyes, allowing digested DNA samples to be loaded directly onto agarose gels without adding a separate loading dye. In a 1% agarose gel, the red dye migrates similarly to approximately 2,500 bp double-stranded DNA, while the yellow dye migrates similarly to approximately 10 bp double-stranded DNA.

Velocut RsaI cannot be heat-inactivated. When enzyme removal is required before downstream applications, phenol-chloroform extraction or column purification is recommended. Cleavage of CpG-methylated DNA may be inhibited, so DNA methylation status should be considered when planning digestion.

Product Features

• Rapid DNA Digestion: Completes digestion in 5–15 minutes at 37°C, supporting efficient restriction analysis of plasmid DNA, PCR products, and genomic DNA containing the target recognition site.
• Convenient Gel Electrophoresis: The supplied color reaction buffer contains red and yellow tracking dyes, enabling direct gel loading and convenient monitoring of electrophoresis progress.
• Defined Methylation Compatibility: CpG methylation may inhibit DNA cleavage. The enzyme cannot be heat-inactivated, so appropriate purification should be considered when enzyme removal is necessary for downstream workflows.

FAQ