Product Overview
Velocut SbfI is a recombinant restriction endonuclease designed for rapid DNA digestion in molecular biology workflows. It is suitable for plasmid DNA, PCR products, and genomic DNA containing the SbfI recognition site. Under the recommended conditions with 1× reaction buffer at 37°C, digestion can typically be completed in 5–15 minutes.
The supplied color reaction buffer contains red and yellow tracking dyes, allowing digested DNA samples to be loaded directly onto agarose gels without adding a separate loading dye. In a 1% agarose gel, the red dye migrates similarly to approximately 2,500 bp double-stranded DNA, while the yellow dye migrates similarly to approximately 10 bp double-stranded DNA.
After digestion, Velocut SbfI can be heat-inactivated at 80°C for 20 minutes, providing a convenient option for workflows that require enzyme inactivation before downstream processing. Store at −20°C.
Product Features
- Rapid DNA Digestion: Supports DNA digestion in 5–15 minutes at 37°C, helping streamline restriction workflows involving plasmid DNA, PCR products, and genomic DNA containing the target recognition site.
- Direct Gel Loading: The supplied color reaction buffer contains red and yellow tracking dyes, enabling direct agarose gel loading and convenient monitoring of electrophoresis progress.
- Convenient Heat Inactivation: Can be heat-inactivated at 80°C for 20 minutes, supporting downstream workflows that benefit from enzyme inactivation after digestion.
FAQ
Q1: How long does Velocut SbfI take to digest DNA?
Under the recommended conditions with 1× reaction buffer at 37°C, Velocut SbfI typically completes digestion in 5–15 minutes. Follow the recommended rapid digestion protocol for reaction setup.
Q2: Can Velocut SbfI be heat-inactivated after digestion?
Yes. Velocut SbfI can be heat-inactivated by incubation at 80°C for 20 minutes.
Q3: Can the digested DNA be loaded directly onto an agarose gel?
Yes. The supplied color reaction buffer contains red and yellow tracking dyes, allowing the reaction mixture to be loaded directly onto an agarose gel without adding a separate loading dye.
