Product Overview
Velocut SdaI (SbfI) is a recombinant restriction endonuclease designed for rapid DNA digestion in molecular biology workflows. It is suitable for plasmid DNA, PCR products, and genomic DNA containing the target recognition site. Under the recommended conditions with 1× reaction buffer at 37°C, digestion can typically be completed in 5–15 minutes.
The supplied color reaction buffer contains red and yellow tracking dyes, allowing digested DNA samples to be loaded directly onto agarose gels without adding a separate loading dye. In a 1% agarose gel, the red dye migrates similarly to approximately 2,500 bp double-stranded DNA, while the yellow dye migrates similarly to approximately 10 bp double-stranded DNA.
Velocut SdaI (SbfI) can be heat-inactivated at 80°C for 20 minutes, supporting convenient processing after restriction digestion. Store at −20°C.
Product Features
- Rapid DNA Digestion: Enables DNA digestion in 5–15 minutes at 37°C, supporting efficient workflows involving plasmid DNA, PCR products, and genomic DNA containing the target recognition site.
- Direct Gel Loading: The supplied color reaction buffer contains red and yellow tracking dyes, enabling direct agarose gel loading and convenient monitoring of electrophoresis progress.
- Convenient Heat Inactivation: Can be heat-inactivated at 80°C for 20 minutes, simplifying post-digestion processing in molecular biology workflows.
FAQ
Q1: How long does Velocut SdaI (SbfI) take to digest DNA?
Under the recommended conditions with 1× reaction buffer at 37°C, Velocut SdaI (SbfI) typically completes digestion in 5–15 minutes. Follow the recommended rapid digestion protocol for reaction setup.
Q2: Can Velocut SdaI (SbfI) be heat-inactivated after digestion?
Yes. Velocut SdaI (SbfI) can be heat-inactivated by incubation at 80°C for 20 minutes.
Q3: Can the digestion mixture be loaded directly onto an agarose gel?
Yes. The supplied color reaction buffer contains red and yellow tracking dyes, allowing the reaction mixture to be loaded directly onto an agarose gel without adding a separate loading dye.
