Product Overview
HYF225 Velocut AflII is a recombinant restriction endonuclease developed for rapid and efficient DNA digestion. Under recommended reaction conditions, it can complete digestion within 5–15 minutes at 37°C, helping streamline molecular biology workflows.
HYF225 Velocut AflII is suitable for digesting DNA substrates containing the appropriate AflII recognition site, including plasmid DNA, PCR products, and genomic DNA. It is compatible with standard reaction buffer and color reaction buffer. The color buffer contains red and yellow tracking dyes, allowing digestion products to be loaded directly onto an agarose gel without adding a separate loading dye. On a 1% agarose gel, the red and yellow dyes migrate similarly to approximately 2,500 bp and 10 bp double-stranded DNA fragments, respectively.
The enzyme can be heat-inactivated at 80°C for 20 minutes. For product stability, store HYF225 Velocut AflII at −20°C. The stated shelf life is two years under the recommended storage conditions.
Product Features
- Rapid DNA Digestion: Completes DNA digestion in 5–15 minutes at 37°C under recommended reaction conditions, helping improve workflow efficiency.
- Convenient Gel Loading: Compatible with color reaction buffer containing red and yellow tracking dyes, enabling direct loading of digestion products onto agarose gels without a separate loading dye.
- Defined Inactivation and Storage Conditions: Can be heat-inactivated at 80°C for 20 minutes. Store at −20°C, with a stated shelf life of two years under the recommended storage conditions.
FAQ
Q1: How long does HYF225 Velocut AflII take to digest DNA?
Under recommended reaction conditions, HYF225 Velocut AflII can complete DNA digestion in 5–15 minutes at 37°C. Actual digestion performance may depend on the DNA substrate and reaction setup.
Q2: Can digestion products generated by HYF225 Velocut AflII be loaded directly onto an agarose gel?
Yes. When using a compatible color reaction buffer, the included tracking dyes allow digestion products to be loaded directly onto an agarose gel without adding a separate loading dye.
Q3: How should HYF225 Velocut AflII be stored, and how can it be inactivated?
Store HYF225 Velocut AflII at −20°C. Its stated shelf life is two years under the recommended storage conditions. For heat inactivation, incubate the reaction at 80°C for 20 minutes.
