Product Overview
HYF227 Velocut AluI is a recombinant restriction endonuclease designed for rapid and efficient DNA digestion. Under recommended reaction conditions, it can complete digestion within 5–15 minutes at 37°C, helping streamline molecular biology workflows.
HYF227 Velocut AluI is suitable for digesting DNA substrates containing the appropriate AluI recognition site, including plasmid DNA, PCR products, and genomic DNA. It is compatible with standard reaction buffer and color reaction buffer. The color buffer contains red and yellow tracking dyes, allowing digestion products to be loaded directly onto an agarose gel without adding a separate loading dye. On a 1% agarose gel, the red and yellow dyes migrate similarly to approximately 2,500 bp and 10 bp double-stranded DNA fragments, respectively.
The enzyme can be heat-inactivated at 80°C for 20 minutes. Digestion of EcoBI-methylated DNA may be inhibited, so DNA methylation status should be considered when planning experiments. Store HYF227 Velocut AluI at −20°C. The stated shelf life is two years under the recommended storage conditions.
Product Features
- Rapid DNA Digestion: Completes DNA digestion in 5–15 minutes at 37°C under recommended reaction conditions, helping improve workflow efficiency.
- Convenient Gel Loading: Compatible with color reaction buffer containing red and yellow tracking dyes, enabling direct loading of digestion products onto agarose gels without a separate loading dye.
- Defined Inactivation and Storage Conditions: Can be heat-inactivated at 80°C for 20 minutes. Store at −20°C, with a stated shelf life of two years under the recommended storage conditions. Digestion of EcoBI-methylated DNA may be inhibited.
FAQ
Q1: How long does HYF227 Velocut AluI take to digest DNA?
Under recommended reaction conditions, HYF227 Velocut AluI can complete DNA digestion in 5–15 minutes at 37°C. Actual digestion performance may depend on the DNA substrate and reaction setup.
Q2: Does DNA methylation affect HYF227 Velocut AluI activity?
Yes. Digestion of EcoBI-methylated DNA may be inhibited. Consider the methylation status of the target DNA when planning your experiment.
Q3: How should HYF227 Velocut AluI be stored and inactivated?
Store HYF227 Velocut AluI at −20°C. Its stated shelf life is two years under the recommended storage conditions. For heat inactivation, incubate the reaction at 80°C for 20 minutes.
