Product Overview
HYF231 Velocut AseI is a recombinant restriction endonuclease developed for rapid and efficient DNA digestion. Under recommended reaction conditions, it can complete digestion within 5–15 minutes at 37°C, helping streamline molecular biology workflows.
HYF231 Velocut AseI is suitable for digesting DNA substrates containing the appropriate AseI recognition site, including plasmid DNA, PCR products, and genomic DNA. It is compatible with standard reaction buffer and color reaction buffer. The color buffer contains red and yellow tracking dyes, allowing digestion products to be loaded directly onto an agarose gel without adding a separate loading dye. On a 1% agarose gel, the red and yellow dyes migrate similarly to approximately 2,500 bp and 10 bp double-stranded DNA fragments, respectively.
The enzyme can be heat-inactivated at 80°C for 20 minutes. Cleavage of EcoBI-methylated DNA may be inhibited, so DNA methylation status should be considered when planning digestion reactions. Store HYF231 Velocut AseI at −20°C. The stated shelf life is two years under the recommended storage conditions.
Product Features
- Rapid DNA Digestion: Completes DNA digestion in 5–15 minutes at 37°C under recommended reaction conditions, supporting efficient restriction digestion workflows.
- Convenient Gel Loading: Compatible with color reaction buffer containing red and yellow tracking dyes, allowing digestion products to be loaded directly onto agarose gels without a separate loading dye.
- Defined Heat Inactivation: Can be heat-inactivated at 80°C for 20 minutes. Store at −20°C, with a stated shelf life of two years under the recommended storage conditions. Cleavage of EcoBI-methylated DNA may be inhibited.
FAQ
Q1: How long does HYF231 Velocut AseI take to digest DNA?
Under recommended reaction conditions, HYF231 Velocut AseI can complete DNA digestion in 5–15 minutes at 37°C. Actual digestion performance may depend on the DNA substrate and reaction setup.
Q2: Can DNA methylation affect HYF231 Velocut AseI activity?
Yes. Cleavage of EcoBI-methylated DNA may be inhibited. Consider the methylation status of the target DNA when planning your digestion experiment.
Q3: How should HYF231 Velocut AseI be stored and inactivated?
Store HYF231 Velocut AseI at −20°C. Its stated shelf life is two years under the recommended storage conditions. For heat inactivation, incubate the reaction at 80°C for 20 minutes.
