Product Overview
HYF235 Velocut BbsI is a recombinant restriction endonuclease developed for rapid and efficient DNA digestion. It can digest DNA containing the appropriate BbsI recognition site, including plasmid DNA, PCR products, and genomic DNA.
Under the recommended reaction conditions, digestion can be completed in 5–15 minutes at 37°C. The enzyme is compatible with standard reaction buffer and color reaction buffer. The color buffer contains red and yellow tracking dyes, allowing digested samples to be loaded directly onto an agarose gel without adding a separate loading dye. In a 1% agarose gel, the red dye migrates similarly to approximately 2,500 bp double-stranded DNA, while the yellow dye migrates similarly to approximately 10 bp double-stranded DNA.
HYF235 Velocut BbsI is insensitive to Dam, Dcm, and CpG methylation and can be heat-inactivated at 80°C for 20 minutes. Store at −20°C for up to 2 years.
Product Features
- Rapid DNA Digestion: Completes restriction digestion in 5–15 minutes at 37°C under recommended conditions.
- Convenient Gel Loading: Color reaction buffer contains tracking dyes for direct loading of digested samples onto agarose gels.
- Broad Methylation Tolerance: Insensitive to Dam, Dcm, and CpG methylation; heat-inactivatable at 80°C for 20 minutes.
FAQ
Q1: How long does HYF235 Velocut BbsI take to digest DNA?
Under the recommended reaction conditions, HYF235 Velocut BbsI typically completes digestion in 5–15 minutes at 37°C. Actual performance may depend on the DNA substrate and reaction setup.
Q2: Is HYF235 Velocut BbsI affected by DNA methylation?
HYF235 Velocut BbsI is insensitive to Dam, Dcm, and CpG methylation according to the product specifications.
Q3: Can HYF235 Velocut BbsI be heat-inactivated after digestion?
Yes. HYF235 Velocut BbsI can be heat-inactivated by incubation at 80°C for 20 minutes.
