Product Overview
HYF237 Velocut BglI is a recombinant restriction endonuclease designed for rapid and efficient DNA digestion in molecular biology workflows. It can be used to digest DNA containing the appropriate BglI recognition site, including plasmid DNA, PCR products, and genomic DNA.
Under the recommended reaction conditions, digestion can be completed in 5–15 minutes at 37°C. The enzyme is compatible with standard reaction buffer and color reaction buffer. The color reaction buffer contains red and yellow tracking dyes, allowing digested samples to be loaded directly onto an agarose gel without adding a separate loading dye. In a 1% agarose gel, the red dye migrates similarly to approximately 2,500 bp double-stranded DNA, while the yellow dye migrates similarly to approximately 10 bp double-stranded DNA.
Cleavage by HYF237 Velocut BglI may be inhibited by CpG methylation. The enzyme can be heat-inactivated at 80°C for 20 minutes.
Product Features
- Rapid DNA Digestion: Enables DNA digestion in 5–15 minutes at 37°C under recommended reaction conditions.
- Convenient Gel Analysis: Color reaction buffer contains tracking dyes, allowing digested samples to be loaded directly onto agarose gels.
- Defined Heat Inactivation: Can be heat-inactivated at 80°C for 20 minutes; CpG methylation may inhibit DNA cleavage.
FAQ
Q1: How long does HYF237 Velocut BglI take to digest DNA?
HYF237 Velocut BglI can complete digestion in 5–15 minutes at 37°C under the recommended reaction conditions. Actual performance may depend on the DNA substrate and reaction setup.
Q2: Does CpG methylation affect HYF237 Velocut BglI activity?
Yes. Cleavage may be inhibited by CpG methylation. Consider the methylation status of your DNA when planning a digestion reaction.
Q3: Can HYF237 Velocut BglI be heat-inactivated after digestion?
Yes. HYF237 Velocut BglI can be heat-inactivated by incubation at 80°C for 20 minutes.
