Product Overview
HYF241 Velocut BspHI is a recombinant restriction endonuclease designed for rapid DNA digestion in molecular biology workflows. It can be used to digest DNA containing the appropriate BspHI recognition site, including plasmid DNA, PCR products, and genomic DNA.
Under the recommended reaction conditions, digestion can be completed in 5–15 minutes at 37°C. The enzyme is compatible with standard reaction buffer and color reaction buffer. The color reaction buffer contains red and yellow tracking dyes, allowing digested samples to be loaded directly onto an agarose gel without adding a separate loading dye. In a 1% agarose gel, the red dye migrates similarly to approximately 2,500 bp double-stranded DNA, while the yellow dye migrates similarly to approximately 10 bp double-stranded DNA.
Cleavage by HYF241 Velocut BspHI may be inhibited by Dam or EcoBI methylation. The enzyme can be heat-inactivated at 80°C for 20 minutes.
Product Features
- Rapid DNA Digestion: Enables DNA digestion in 5–15 minutes at 37°C under recommended reaction conditions.
- Convenient Gel Analysis: Color reaction buffer contains tracking dyes, allowing digested samples to be loaded directly onto agarose gels.
- Defined Heat Inactivation: Can be heat-inactivated at 80°C for 20 minutes and stored at −20°C for up to 2 years. EcoBI methylation may inhibit cleavage.
FAQ
Q1: How long does HYF241 Velocut BspHI take to digest DNA?
HYF241 Velocut BspHI can complete digestion in 5–15 minutes at 37°C under the recommended reaction conditions. Actual performance may depend on the DNA substrate and reaction setup.
Q2: Can DNA methylation affect HYF241 Velocut BspHI activity?
Yes. Cleavage may be inhibited by Dam or EcoBI methylation. Consider the methylation status of your DNA when planning a digestion reaction.
Q3: Can HYF241 Velocut BspHI be heat-inactivated after digestion?
Yes. HYF241 Velocut BspHI can be heat-inactivated by incubation at 80°C for 20 minutes.
