Product Overview
HYF242 Velocut BsrGI is a recombinant restriction endonuclease designed for rapid DNA digestion in molecular biology workflows. It can be used to digest DNA containing the appropriate BsrGI recognition site, including plasmid DNA, PCR products, and genomic DNA.
Under the recommended reaction conditions, digestion can be completed in 5–15 minutes at 37°C. The enzyme is compatible with standard reaction buffer and color reaction buffer. The color reaction buffer contains red and yellow tracking dyes, allowing digested samples to be loaded directly onto an agarose gel without adding a separate loading dye. In a 1% agarose gel, the red dye migrates similarly to approximately 2,500 bp double-stranded DNA, while the yellow dye migrates similarly to approximately 10 bp double-stranded DNA.
HYF242 Velocut BsrGI can be heat-inactivated at 80°C for 20 minutes and stored at −20°C for up to 2 years.
Product Features
- Rapid DNA Digestion: Enables DNA digestion in 5–15 minutes at 37°C under recommended reaction conditions.
- Convenient Gel Analysis: Color reaction buffer contains tracking dyes, allowing digested samples to be loaded directly onto agarose gels.
- Convenient Inactivation and Storage: Can be heat-inactivated at 80°C for 20 minutes and stored at −20°C for up to 2 years.
FAQ
Q1: How long does HYF242 Velocut BsrGI take to digest DNA?
HYF242 Velocut BsrGI can complete digestion in 5–15 minutes at 37°C under the recommended reaction conditions. Actual performance may depend on the DNA substrate and reaction setup.
Q2: Can HYF242 Velocut BsrGI be heat-inactivated after digestion?
Yes. HYF242 Velocut BsrGI can be heat-inactivated by incubation at 80°C for 20 minutes.
Q3: How should HYF242 Velocut BsrGI be stored?
Store HYF242 Velocut BsrGI at −20°C. The specified storage period is up to 2 years.
