Product Overview
HYF243 Velocut BstBI is a recombinant restriction endonuclease designed for rapid DNA digestion in molecular biology workflows. It can be used to digest DNA containing the appropriate BstBI recognition site, including plasmid DNA, PCR products, and genomic DNA.
Under the recommended reaction conditions, digestion can be completed in 5–15 minutes at 37°C. The enzyme is compatible with standard reaction buffer and color reaction buffer. The color reaction buffer contains red and yellow tracking dyes, allowing digested samples to be loaded directly onto an agarose gel without adding a separate loading dye. In a 1% agarose gel, the red dye migrates similarly to approximately 2,500 bp double-stranded DNA, while the yellow dye migrates similarly to approximately 10 bp double-stranded DNA.
Cleavage by HYF243 Velocut BstBI may be inhibited by CpG or EcoKI methylation. The enzyme can be heat-inactivated at 80°C for 20 minutes and stored at −20°C for up to 2 years.
Product Features
- Rapid DNA Digestion: Enables DNA digestion in 5–15 minutes at 37°C under recommended reaction conditions.
- Convenient Gel Analysis: Color reaction buffer contains tracking dyes for direct loading of digested samples onto agarose gels.
- Defined Inactivation and Storage: Can be heat-inactivated at 80°C for 20 minutes and stored at −20°C for up to 2 years. CpG or EcoKI methylation may inhibit cleavage.
FAQ
Q1: How long does HYF243 Velocut BstBI take to digest DNA?
HYF243 Velocut BstBI can complete digestion in 5–15 minutes at 37°C under the recommended reaction conditions. Actual performance may depend on the DNA substrate and reaction setup.
Q2: Can DNA methylation affect HYF243 Velocut BstBI activity?
Yes. Cleavage may be inhibited by CpG or EcoKI methylation. Consider the methylation status of your DNA when planning a digestion reaction.
Q3: How should HYF243 Velocut BstBI be inactivated and stored?
HYF243 Velocut BstBI can be heat-inactivated at 80°C for 20 minutes. Store the enzyme at −20°C for up to 2 years.
