Product Overview
HYF253 Velocut EarI is a recombinant restriction endonuclease developed for efficient DNA digestion in molecular biology workflows. It is suitable for digesting plasmid DNA, PCR products, and genomic DNA containing the appropriate EarI recognition site. Under recommended reaction conditions, digestion can typically be completed in 5–15 minutes at 37°C.
For convenient downstream analysis, a color reaction buffer containing red and yellow tracking dyes allows digestion reactions to be loaded directly onto an agarose gel without adding a separate loading dye. In a 1% agarose gel, the red dye migrates similarly to approximately 2,500 bp double-stranded DNA, while the yellow dye migrates similarly to approximately 10 bp double-stranded DNA.
Cleavage of CpG-methylated DNA may be inhibited, so the methylation status of the DNA substrate should be considered when planning digestion. HYF253 Velocut EarI can be heat-inactivated at 80°C for 20 minutes and should be stored at −20°C, with a shelf life of two years.
Product Features
- Rapid DNA Digestion: Supports restriction digestion in 5–15 minutes at 37°C under recommended reaction conditions.
- Convenient Gel Electrophoresis: A color reaction buffer with red and yellow tracking dyes enables direct loading of digestion reactions onto agarose gels.
- Defined Inactivation and Storage Conditions: Can be heat-inactivated at 80°C for 20 minutes and stored at −20°C for two years.
FAQ
Q1: How long does HYF253 Velocut EarI take to digest DNA?
A: Under the recommended reaction conditions, digestion is typically completed in 5–15 minutes at 37°C.
Q2: Does CpG methylation affect HYF253 Velocut EarI activity?
A: Cleavage of CpG-methylated DNA may be inhibited. Consider the methylation status of your DNA substrate when planning the digestion workflow.
Q3: How should HYF253 Velocut EarI be inactivated and stored?
A: The enzyme can be heat-inactivated at 80°C for 20 minutes. Store at −20°C; the stated shelf life is two years.
