Product Overview
HYF272 Velocut MseI is a rapid restriction enzyme designed to streamline DNA digestion in molecular biology workflows. Under the recommended reaction conditions, it can digest suitable DNA substrates in 5–15 minutes at 37°C, supporting efficient DNA preparation for downstream applications.
The supplied color reaction buffer contains red and yellow tracking dyes, allowing digested samples to be loaded directly onto an agarose gel without adding a separate loading dye. In a 1% agarose gel, the red dye migrates similarly to approximately 2,500 bp double-stranded DNA, while the yellow dye migrates similarly to approximately 10 bp double-stranded DNA.
Velocut MseI can be heat-inactivated at 80°C for 20 minutes. Cleavage of EcoKI-methylated DNA may be inhibited, so the methylation status of the DNA substrate should be considered when planning digestion workflows. Store at −20°C for up to 2 years.
Product Features
- Rapid DNA Digestion: Enables DNA digestion in 5–15 minutes at 37°C under the recommended reaction conditions, supporting efficient processing of plasmid DNA, PCR products, and genomic DNA containing the recognition site.
- Direct Gel Loading: The supplied color reaction buffer contains red and yellow tracking dyes, allowing digested samples to be loaded directly onto an agarose gel and facilitating electrophoresis monitoring.
- Defined Methylation Sensitivity: Cleavage of EcoKI-methylated DNA may be inhibited. The enzyme can be heat-inactivated at 80°C for 20 minutes, supporting predictable workflow planning.
FAQ
Q1: How long does Velocut MseI digestion take?
Under the recommended reaction conditions, Velocut MseI typically completes DNA digestion in 5–15 minutes at 37°C. Actual performance depends on the DNA substrate and reaction setup.
Q2: Does EcoKI methylation affect Velocut MseI digestion?
Yes. Cleavage of EcoKI-methylated DNA may be inhibited. Consider the methylation status of the DNA substrate when planning the digestion reaction.
Q3: Can Velocut MseI digestion products be loaded directly onto an agarose gel?
Yes. The supplied color reaction buffer contains red and yellow tracking dyes, allowing direct gel loading. In a 1% agarose gel, the red dye migrates similarly to approximately 2,500 bp double-stranded DNA, and the yellow dye migrates similarly to approximately 10 bp double-stranded DNA.
