Product Overview

HYF282 Velocut PmlI is a rapid restriction enzyme designed to support efficient DNA digestion in molecular biology workflows. Under the recommended reaction conditions, it enables digestion in 5–15 minutes at 37°C, helping streamline DNA preparation for downstream applications.

The supplied color reaction buffer contains red and yellow tracking dyes, allowing digestion products to be loaded directly onto an agarose gel without adding a separate loading dye. In a 1% agarose gel, the red dye migrates similarly to approximately 2,500 bp double-stranded DNA, while the yellow dye migrates similarly to approximately 10 bp double-stranded DNA.

Velocut PmlI can be heat-inactivated at 80°C for 20 minutes. Cleavage of CpG-, EcoKI-, or EcoBI-methylated DNA may be inhibited, so the methylation status of the DNA substrate should be considered when planning digestion workflows. Store at −20°C for up to 2 years.

Product Features

• Rapid DNA Digestion: Enables digestion in 5–15 minutes at 37°C under the recommended reaction conditions for suitable plasmid DNA, PCR products, and genomic DNA containing the recognition site.
• Convenient Gel Electrophoresis: The supplied color reaction buffer contains red and yellow tracking dyes, allowing direct gel loading and facilitating monitoring of electrophoresis progress.
• Defined Methylation Sensitivity: Cleavage of CpG-, EcoKI-, or EcoBI-methylated DNA may be inhibited. The enzyme can be heat-inactivated at 80°C for 20 minutes.

FAQ