Product Overview

HYF283 Velocut PspGI (EcoRII) is a rapid restriction enzyme developed for efficient DNA digestion in molecular biology workflows. Under the recommended reaction conditions, it enables digestion in 5–15 minutes at 75°C, supporting DNA preparation for downstream applications.

The supplied color reaction buffer contains red and yellow tracking dyes, allowing digestion products to be loaded directly onto an agarose gel without adding a separate loading dye. In a 1% agarose gel, the red dye migrates similarly to approximately 2,500 bp double-stranded DNA, while the yellow dye migrates similarly to approximately 10 bp double-stranded DNA.

Velocut PspGI (EcoRII) cannot be heat-inactivated. Phenol-chloroform extraction or column purification is recommended when enzyme removal is required after digestion. Cleavage of Dcm-methylated DNA may be inhibited, so the methylation status of the DNA substrate should be considered during experimental planning. Store at −20°C for up to 2 years.

Product Features

• Rapid Digestion at 75°C: Supports DNA digestion in 5–15 minutes at the recommended temperature of 75°C under the specified reaction conditions.
• Convenient Direct Gel Loading: The supplied color reaction buffer contains red and yellow tracking dyes, enabling direct loading of digestion products onto agarose gels and facilitating electrophoresis monitoring.
• Defined Methylation Sensitivity and Enzyme Removal: Cleavage of Dcm-methylated DNA may be inhibited. The enzyme cannot be heat-inactivated; phenol-chloroform extraction or column purification is recommended when enzyme removal is required.

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