Product Overview
SgeI (HYF213) is a restriction endonuclease designed to recognize and cleave specific DNA targets containing 5-methylcytosine (5-mC). It recognizes the ᵐ⁵CNNG(9/13)^ recognition site and can act on target DNA in either single-stranded or double-stranded form.
For optimal performance, SgeI is used with the recommended 1× reaction buffer and incubated at 37°C. The enzyme’s storage buffer contains premixed bovine serum albumin (BSA), which helps stabilize the enzyme and bind potential contaminants present in DNA preparations. SgeI can be heat-inactivated at 65°C for 5 minutes.
Product Features
- 5-Methylcytosine-Containing DNA Recognition: Recognizes and cleaves DNA targets containing 5-methylcytosine in single- or double-stranded DNA.
- Defined Recognition Site: Recognizes the ᵐ⁵CNNG(9/13)^ site for targeted DNA cleavage.
- Convenient Reaction and Inactivation Conditions: Performs optimally at 37°C in the recommended reaction buffer and can be heat-inactivated at 65°C for 5 minutes.
FAQ
Q1: What DNA targets does SgeI recognize?
SgeI recognizes the ᵐ⁵CNNG(9/13)^ site in DNA targets containing 5-methylcytosine and can cleave targets in either single-stranded or double-stranded DNA.
Q2: What are the recommended reaction conditions for SgeI?
Use the recommended 1× SgeI reaction buffer and incubate at 37°C. Prepare the reaction according to the applicable DNA digestion protocol.
Q3: How can SgeI be heat-inactivated after digestion?
SgeI can be heat-inactivated by incubating the reaction at 65°C for 5 minutes.
