Product Overview
BbvCI (HYF413) is a recombinant restriction endonuclease used for sequence-specific DNA cleavage in molecular biology workflows. It recognizes the non-palindromic sequence CCTCAGC and cleaves DNA to produce 5′ overhangs with three nucleotides, making it suitable for applications that require defined sticky-end DNA fragments.
BbvCI is compatible with the recommended 1× reaction buffer and can be used for double digestion with other compatible restriction enzymes. Incubation at 37°C is recommended. Prolonged digestion exceeding 3 hours should be avoided to reduce the risk of star activity.
The supplied color reaction buffer contains red and yellow tracking dyes, allowing digestion products to be loaded directly onto an agarose gel without adding a separate loading dye. In a 1% agarose gel, the red dye migrates similarly to a 2,500 bp double-stranded DNA fragment, while the yellow dye migrates similarly to a 10 bp fragment. BbvCI can be heat-inactivated at 65°C for 5 minutes. Cleavage of CpG-methylated DNA may be inhibited.
Product Features
- Sequence-Specific DNA Cleavage: Recognizes the non-palindromic sequence CCTCAGC and generates 5′ overhangs with three nucleotides.
- Compatible Double Digestion: Supports double-digestion workflows with other compatible restriction enzymes under recommended reaction conditions.
- Convenient Workflow Control: Enables direct gel loading with the supplied color reaction buffer and can be heat-inactivated at 65°C for 5 minutes. Digestion longer than 3 hours should be avoided to reduce the risk of star activity.
FAQ
Q1: What DNA sequence does BbvCI recognize, and what ends does it generate?
BbvCI recognizes the non-palindromic sequence CCTCAGC and generates 5′ overhangs with three nucleotides.
Q2: Can BbvCI be used for double digestion with another restriction enzyme?
Yes. BbvCI can be used in double-digestion workflows with compatible restriction enzymes. Confirm buffer compatibility and follow the recommended reaction conditions for both enzymes.
Q3: Does CpG methylation affect BbvCI digestion?
Cleavage of CpG-methylated DNA may be inhibited. Consider the methylation status of the DNA substrate when planning the digestion workflow. Avoid incubations longer than 3 hours to reduce the risk of star activity.
