2×HiF Taq plus Master Mix

Cat No: HCR2014B

HIF Taq plus Master Mix (With Dye) is a ready-to-use 2×premixed solution containing Plus HIF DNA Polymerase, dNTPs, and optimized buffer. Two monoclonal antibodies at room temperature that inhibit polymerase activity and 3′→5′exonuclease activity are added to the master mix for easily and highly specific Hot Start PCR. The extension factor is added to the master mix to give the enzyme a long fragment amplification capacity, the length of the amplification can be up to 13 kb, the enzyme has a 5′→3′ DNA polymerase activity and a 3′→5′ exonuclease activity, its fidelity is 83 times that of Taq DNA polymerase,which is 9 times that of ordinary DNA polymerase. It is suitable for amplification of complex templates, the amplification product is a blunt end.

2×HIF Taq plus Master Mix(With Dye) has the advantages of fast and easy, high sensitivity,strong specificity, good stability, etc., the reaction system only needs to add primers and templates, and can be amplified by a two-step protocol, simplifying the experimental steps and saving time. This product contains electrophoresis indicator dyes, and PCR products can be used directly for electrophoresis. In addition,the product also contains a specific protective agent, so that the master mix can maintain stable activity after repeated freeze-thaw.

Storage Conditions

Products should be stored at -25~-15℃ for 1 year.

Specifications

Instructions

1. PCR Reaction System

2. Recommended usage of different templates

3. Amplification Protocol

1) Two-Step Protocol (complexity template)

2) Three-Step Protocol (regular protocol)

3) Annealing Gradient Protocol (complexity template)

Features under different amplification protocol

Notes

Please wear the necessary PPE, such lab coat and gloves,to ensure your health and safety!