
Fast Ampli RT-qPCR Premix plus-UNG
Cat No: HCB5144E
Fast Ampli RT-qPCR Premix plus-UNG is developed specifically for lyophilization processes, applied for fluorescence quantification (TaqMan probe) RNA amplification detection containing neocript reverse transcriptase and rapid amplification DNA Polymerase obtained by genetically modified and screening which can complete the PCR amplification within 20-40 minutes. This reagent preforms high inhibitor- tolerance, which has higher reverse transcription and PCR amplification efficiency, suitable for high-sensitivity amplification of low-concentration RNA samples. A good standard curve can be obtained within a wide quantitative range, and quantification can be carried out accurately. This reagent uses a mixed enzyme of anti-inhibition amplification enzyme and UNG enzyme, as well as an optimized buffer system containing dUTP. It can not only achieve good amplification of the target gene in samples containing inhibitors, but also effectively prevent false positive amplification caused by PCR residual and aerosol pollution. This reagent is compatible with most fluorescent quantitative PCR instruments, such as Applied Biosystems, Eppendorf, Bio-Rad, Roche and so on. This reagent can be used for lyophilization to obtain a good lyophilized form and product stability.
Storage Conditions
Composition |
Volume (20 μL reaction)
|
FastAmpli RT-qPCR Premix-UNG (Lyophilized
powder)
|
4μL
|
25X Primer Probe Mix 1,2
|
1μl
|
Template RNA3
|
X μL
|
ddH2O |
up to 25μL |
Cycle step
|
Temp.
|
Time
|
Cycles
|
Reverse Transcription |
50℃ |
10-20 min |
1 |
Initial Denaturation
|
95℃ |
1-5 min |
1 |
Denaturation
|
95℃ |
10-20 s |
40-45 |
Annealing/Extension |
56-64℃ |
20-60 s |
40-45 |
Cycle step | Temp. |
Time
|
Cycles
|
Reverse Transcription |
50℃ |
5 min |
1 |
Initial Denaturation |
95℃ |
30 s |
1 |
Denaturation |
95℃ |
1-3 s |
40-45 |
Annealing/Extension |
56-64℃ |
3-20 s |
40-45 |