Hotstart Taq DNA Polymerase M2

Superstart Taq DNA Polymerase is a hot start DNA polymerase. This product can not only better inhibit the non-specific reaction caused by the non-specific annealing of primers or primer aggregation in the process of PCR system preparation and amplification. Therefore, it can achieve better results in multiple amplification. Also, it can optimize the amplification of low concentration templates to obtain a higher amount of product and achieve a more stable and extreme amplification effect.

Components

1.5 U/μL Superstart Taq DNA polymerase

2.10 × PCR Buffer II (Mg²+ free) (optional)

3.25 mM MgCl₂ (optional)

* 10 × PCR Buffer II (Mg²+ free) does not contain dNTP and Mg²+, please add dNTPs and MgCl₂ when preparing the reaction system.

Recommended Applications

1.African swine fever detection.

2.STI detection.

3.Multiplex amplification.

Storage Condition

-20°C for long term storage, should be mixed well before use, avoid frequent freeze-thaw.

*If precipitation occurs after refrigeration, it is normal; it is recommended to equilibrate to room temperature before mixing and use.

Unit Definition

One active unit (U) is defined as the amount of enzyme that incorporate 10 nmol of deoxyribonucleotide into acid-insoluble material at 74°C for 30mins using activated salmon sperm DNA as template/primer.

Quality Control

1.SDS-PAGE electrophoretic purity greater than 98%.

2.Amplification sensitivity, batch-to-batch control, stability.

3.No exogenous nuclease activity, no exogenous endonuclease or exonuclease contamination

Instructions

Reaction Setup

Notes:

1) a. The buffer does not contain dNTP and Mg²+, please add dNTPs and MgCl₂ when preparing the reaction system.

2) b.If used for qPCR/qRT-PCR, fluorescent probes should be added to the reaction system. Usually, a final primer concentration of 0.2 μM will give good results; if the reaction performance is poor, the primer concentration can be adjusted in the range of 0.2-1 μM. The probe concentration is

3) usually optimized in the range of 0.1-0.3 μM. Concentration gradient experiments can be performed to find the best combination of primer and probe.

Thermal cycling protocol

Notes

1.It can adopt 95℃ or 94℃, 1~5 mins rapid hot start.

2.The system is highly adaptable, with higher specificity and sensitivity.

3.In normal PCR, it can obtain a larger amount of product, and in fluorescence quantitative PCR, it can improve the normalization of the amplification curve and the fluorescence value of the template with very low concentration. Suitable for use as a high sensitivity PCR detection reagent.

4.and can be used in multiplex PCR amplification reactions.

5.5′→3′ polymerase activity,5′→3′ exonuclease activity; no 3′→5′ exonuclease activity; no proofreading function.

6.Suitable for qualitative and quantitative testing of PCR and RT-PCR.

7.The 3′ end of the PCR product is A, which can be directly cloned into a T vector.

8.The three-step method is recommended for primers with low annealing temperatures or for amplification of fragments longer than 200 bp.