Bst 2.0 DNA Polymerase (Glycerol free, high density)
Bst DNA polymerase V2 is derived from Bacillus stearothermophilus DNA Polymerase I, which has 5′→3′ DNA polymerase activity and strong chain replacement activity, but no 5′→3′ exonuclease activity. Bst DNA Polymerase V2 is ideally suitable for strand-displacement, isothermal amplification LAMP (Loop mediated isothermal amplification) and rapid sequencing. This Bst DNA polymerase V2 is capable of inhibiting DNA polymerase activity at room temperature, so that it can be operated and the reaction system can be formulated at room temperature, preventing non-specific amplification and improving the efficiency of the reaction, and this version can be lyophilized. In addition, it is capable of releasing its activity at elevated temperatures, thus eliminating the need for a separate activation step.
Components
|
Component |
HC5007A-01 |
HC5007A-02 |
HC5007A-03 |
|
Bst DNA polymerase V2 (Glycerol-free) (32U/μL) |
0.05 mL |
0.25 mL |
2.5 mL |
|
10×HC Bst V2 Buffer |
1.5 mL |
2×1.5 mL |
3×10 mL |
|
MgSO4 (100mM) |
1.5 mL |
2×1.5 mL |
2×10 mL |
Applications
1.LAMP isothermal amplification
2.DNA strand single displacement reaction
3.High GC gene sequencing
4.DNA sequencing of nanogram level.
Storage Condition
Transportation under 0°C and be stored at -25°C~-15°C.
Unit Definition
One unit is defined as the amount of enzyme that incorporate 25 nmol of dNTP into acid insoluble material in 30 minutes at 65°C.
LAMP Reaction
|
Components |
25 μL System |
|
10×HC Bst V2 Buffer |
2.5 μL |
|
MgSO4 (100mM) |
1.5 μL |
|
dNTPs (10mM each) |
3.5 μL |
|
SYTO™ 16 Green (25×) a |
1.0 μL |
|
Primer mix b |
6 μL |
|
Bst DNA Polymerase V2 (Glycerol-free) (32 U/uL) |
0.25 μL |
|
Template |
× μL |
|
ddH₂O |
Up to 25 μL |
Notes:
1) a. SYTOTM 16 Green (25×): According to experimental needs, other dyes can be used as substitutes;
2) b. Primer mix: obtained by mixing 20 µM FIP, 20 µM BIP, 2.5 µM F3, 2.5 µM B3, 5 µM LF, 5 µM LB and other volumes.
Reaction and Condition
1 × HC Bst V2 Buffer, the incubation temperature is between 60°C and 65°C.
Heat Inactivation
80°C, 20mins.
