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Hot Start Taq DNA Polymerase(Antibody modification) Featured Image
  • Hot Start Taq DNA Polymerase(Antibody modification)
  • Hot Start Taq DNA Polymerase(Antibody modification)

Hot Start Taq DNA Polymerase(Antibody modification)


CAS no.: 9012-90-2  EC no.: 2.7.7.7

Package: 250U,500U, 1000U,10000U, 100000U,25000U,100,000 U

Product Detail

Description

HotStart Taq DNA Polymerase is a hot start DNA polymerase with double blocking by double antibodies independently developed by the company. This product not only blocks the 5'→3' polymerase activity of Taq DNA polymerase, but also blocks the 5'→3' exonuclease activity. Heating for 30 seconds at the pre denaturation temperature can completely inactivate the antibody and release DNA polymerase activity and exonuclease activity. The double blocking characteristic can not only effectively prevent the nonspecific amplification caused by mismatch or primer dimer, but also effectively inhibit the decline of fluorescence signal caused by probe degradation, so as to make the in vitro detection reagent more stable during transportation or use at room temperature.

Chemical structure

Chemical structure

Specification

Test items Results
Protein Purity ≥95%(SDS-PAGE)
Protease Activity   5-7U/μL
Blocking Effect ≥90%
Exonuclease Activity

No detectable  

Nickase Activity

No detectable  

Rnase Activity

No detectable  

Amplification ability  Pass

Applications

PCR amplification

Shipping and Storage

Transportation: Ice packs 

Storage Conditions: Store at -25~-15℃ 

Shelf life: 2 years


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